Resisting Protein but Promoting Cell Adhesion by Choline Phosphate:A Comparative Study with Phosphorylcholine  

作　　者：Fuhui Huang Chunmei Ding Jianshu Li 

机构地区：[1]College of Polymer Science and Engineering,Sichuan University,Chengdu,610065,China [2]State Key Laboratory of Polymer Materials Engineering,Sichuan University,Chengdu,610065,China.

出　　处：《Journal of Bioresources and Bioproducts》2018年第1期3-8,共6页生物质资源与工程（英文）

基　　金：support by the National Natural Science Foundation of China(51503126,21534008,51573110);Scientific Research Foundation for Young Teachers of Sichuan University(2015SCU11005);Miaozi Project in Science and Technology Innovation Program of Sichuan Province(2016087).

摘　　要：Zwitterionic materials are now widely used to fabricate various functionalized surfaces for biomedical applications due to their excellent non-fouling properties.However,a newly-discovered zwitterionic material,choline phosphate(CP),was reported to be cell-adhesive,which makes it different from traditional non-fouling zwitterionic materials such as sulfobetaine,carboxybetaine and phosphorylcholine(PC).To further investigate the properties of CP,a comparative study was conducted and the widely-reported zwitterionic PC was employed as a control which has the same chemical component but opposite orientation of charged groups with CP.For this purpose,CP and PC-functionalized surfaces were prepared by surface-initiated atom transfer radical polymerization(Si-ATRP),and their non-fouling properties were probed by protein adsorption and eukaryotic cell adhesion measurement.Results showed that CP-functionalized surfaces exhibited almost equivalent amounts of adsorbed proteins to that of PC,but they were more beneficial to cells initial adhesion and further spreading in serum-free medium,indicating that CP had a promising prospect of application in tissue engineering.

关 键 词：ZWITTERIONIC Choline phosphate PHOSPHORYLCHOLINE ATRP Non-fouling properties 

分 类 号：O62[理学—有机化学]