槐耳清膏对乳腺癌他莫昔芬耐药细胞的影响及其作用机制  

Effect of Huaier aqueous extract on breast cancer tamoxifenresistant LCC2 cells and its mechanism

作　　者：薛姣姣 郝磊 张毓秀 代贺阳 张丽霞[1] 郭少伟 张晶晶[5] 李阳[1] 李庆霞[1,2] XUE Jiaojiao;HAO Lei;ZHANG Yuxiu;DAI Heyang;ZHANG Lixia;GUO Shaowei;ZHANG Jingjing;LI Yang;LI Qingxia(Fourth Department of Oncology,People’s Hospital,Hebei Province,Shijiazhuang 05000,China;Graduate School,Hebei Medical University,Shijiazhuang 05000,China;Department of Oncology Prevention and Control,Seventh Affiliated Hospital,Southern Medical University,Foshan 528200,China;Graduate School,North China University of Science and Technology,Tangshan 063000,China;Department of Health Service,People’s Hospital,Hebei Province,Shijiazhuang 05000,China)

机构地区：[1]河北省人民医院肿瘤四科,河北石家庄050000 [2]河北医科大学研究生院,河北石家庄050000 [3]南方医科大学第七附属医院肿瘤防治科,广东佛山528200 [4]华北理工大学研究生院,河北唐山063000 [5]河北省人民医院保健处,河北石家庄050000

出　　处：《吉林大学学报（医学版）》2023年第3期714-721,共8页Journal of Jilin University：Medicine Edition

基　　金：河北省科技厅自然科学基金精准医学联合培育项目(H2022307024);河北省卫健委医学科学研究课题计划项目(20210441)。

摘　　要：目的:探讨槐耳清膏(HAE)对乳腺癌他莫昔芬(TAM)耐药LCC2细胞增殖、迁移、细胞周期和凋亡的影响,并阐明其作用机制。方法:细胞分为对照组(给予DMEM基础培养基)、TAM组(给予2μmol·L^(-1)TAM)、TAM+HAE组(给予2μmol·L^(-1)TAM+4 g·L^(-1)HAE)和0、2、4、8及16 g·L^(-1)HAE组。MTT法检测各组细胞存活率,细胞划痕实验检测各组细胞迁移率,流式细胞术检测各组不同细胞周期细胞百分率和细胞凋亡率,Transwell小室实验检测各组迁移细胞数,Western blotting法检测各组细胞中雌激素受体α(ERα)、乳腺癌扩增基因1(AIB1)和survivin蛋白表达水平。结果:MTT法检测,各浓度HAE组作用细胞24 h,与0 g·L^(-1)HAE组比较,4、8和16 g·L^(-1)HAE组细胞存活率明显降低(P<0.01)。作用48 h时,与0 g·L^(-1)HAE组比较,2、4、8和16 g·L^(-1)HAE组细胞存活率明显降低(P<0.01);与4g·L^(-1)HAE组比较,8g.L-1HAE组细胞存活率降低(P<0.05);与作用24 h时比较,作用48 h时各浓度HAE组细胞存活率明显降低(P<0.01)。作用48 h时,与TAM组和4 g·L^(-1)HAE组比较,TAM+HAE组细胞存活率明显降低(P<0.01)。细胞划痕实验,作用细胞48 h时,与0 g·L^(-1)HAE组比较,4 g·L^(-1)HAE组细胞迁移率降低(P<0.05)。流式细胞术检测,作用48 h时,与0g·L^(-1)HAE组比较,4g·L^(-1)HAE组S期细胞百分率和细胞凋亡率明显升高(P<0.05或P<0.01)。Transwell小室实验,作用48 h时,与TAM组和4g·L^(-1)HAE组比较,TAM+HAE组迁移细胞数明显减少(P<0.01)。Western blotting法检测,与MCF-7细胞比较,LCC2细胞中ERα、AIB1和survivin蛋白表达水平明显升高(P<0.05或P<0.01);作用48 h时,与0g·L^(-1)HAE组比较,2、4、8和16 g·L^(-1)HAE组细胞中ERα、AIB1及survivin蛋白表达水平明显降低(P<0.01)。结论:HAE可抑制LCC2细胞的增殖和迁移,阻滞细胞周期于S期并促进细胞凋亡,同时可恢复LCC2细胞对TAM的敏感性,其作用机制可能与下调LCC2细胞中ERα、AIB1和survivin蛋白表达有关�Objective:To discuss the effect of Huaier aqueous extract(HAE)on the proliferation,migration,cell cycle,and apoptosis of tamoxifen(TAM)resistant breast cancer LCC2 cells,and to clarify its mechanism.Methods:The cells were divided into control group(given DMEM basal medium)and TAM group(given 2μmol·L^(-1)TAM),TAM+HAE group(given 2μmol·L^(-1)TAM+4 g·L^(-1)HAE)and 0,2,4,8,and 16 g·L^(-1)HAE groups.MTT method was used to detect the survival rates and the proliferation rates of the cells in various groups;cell scratch test was used to detect the migration rates of the cells in various groups;flow cytometry was used to detect the percentages of the cells at different cell cycles and apoptotic rates of the cells in various groups;Transwell chamber test was used to detect the migration number of the cells in various groups;Western blotting method was used to detect the expression levels of estrogen receptorα(ERα)in each group,amplified in breast cancer 1(AIB1),and survivin proteins in the cells in various groups.Results:The MTT assay showed that after treated for 24 h,compared with 0 g·L^(-1)HAE group,the survival rates of the HAE cells in 4,8,and 16 g·L^(-1)HAE groups were significantly decreased(P<0.01).At 48 h after treatment,compared with 0 g·L^(-1)HAE group,the survival rates of the cells in 2,4,8,and 16 g·L^(-1)HAE groups were significantly decreased(P<0.01);compared with 4 g·L^(-1)HAE group,the survival rate of the cells in 8 g·L^(-1)HAE groups was decreased(P<0.05);compared with 24 h after treatment,the surival rates of the cells in different concentrations of HAE groups were significantly decreased(P<0.01)after treated for 48 h.At 48 treatment,compared with TAM and 4 g·L^(-1)HAE group,the proliferation rate of the cells in TAM+HAE group was significantly decreased(P<0.01).The cell scratch test results showed that the cell migration rate of the cells in 4 g·L^(-1)HAE group was lower than that in 0 g·L^(-1)HAE group after treated for 48 h(P<0.05).The flow cytometry results showed that after treate

关 键 词：乳腺癌 槐耳清膏 他莫昔芬 细胞增殖 耐药 

分 类 号：R737.9[医药卫生—肿瘤]