红细胞来源α-突触核蛋白作为早发型帕金森病生物标记物的研究  

Alpha-Synuclein Species in Red Blood Cells as Biomarkers for Early-onset Parkinson’s Dis⁃ease

作　　者：柳欣[1] 蔡慧慧 郑元初 LIU Xin;CAI Hui-hui;ZHENG Yuan-chu(Center for Movement Disorders,Department of Neurology,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学附属北京天坛医院运动障碍性疾病科,北京100070

出　　处：《神经损伤与功能重建》2023年第6期311-315,共5页Neural Injury and Functional Reconstruction

摘　　要：目的:研究早发型(发病年龄<50岁)帕金森病(PD)患者红细胞中是否存在α-突触核蛋白(α-Syn)异常沉积,以及红细胞来源α-Syn能否作为早发型PD的生物标志物。方法:早发型PD患者26例纳入早发型PD组,30例年龄、性别匹配的健康对照受试者纳入对照组;收集所有入组受试者的人口学及临床资料。对早发型PD组的患者进行国际运动障碍协会统一帕金森病评价量表(MDS-UPDRS)和Hoehn&Yahr(H-Y)量表、简易精神状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)测评。应用电化学发光免疫测定法检测2组红细胞中的α-Syn单体和α-Syn聚集体浓度,并分析早发型PD患者红细胞来源α-Syn单体和α-Syn聚集体浓度与临床指标的相关性。结果:早发型PD患者的α-Syn单体和α-Syn聚集体的水平显著高于健康对照(P=0.009、P<0.0001)。在诊断效力方面,红细胞α-Syn聚集体优于α-Syn单体,红细胞α-Syn聚集体诊断早发型PD的AUC为0.887(95%CI 0.794-0.981),敏感度为73.3%,特异度为96.2%。早发型PD组红细胞来源α-Syn单体和α-Syn聚集体浓度与患者年龄、病程、H-Y分期、MDS-UPDRS-Ⅲ评分、MMSE评分、MOCA评分等指标均无显著相关性(P>0.05)。结论:早发型PD患者红细胞中存在α-Syn异常沉积,红细胞来源α-Syn特别是α-Syn聚集体可作为早发型PD的生物标志物。Objective:To investigate the presence of abnormalα-synuclein(α-Syn)deposition in red blood cells(RBCs)in patients with early-onset Parkinson’s disease(PD)and determine whether RBC-derivedα-Syn could serve as a biomarker for early-onset PD.Methods:A total of 26 patients with early-onset PD(onset age<50 years)were included in the early-onset PD group,and 30 age-and sex-matched healthy control participants were included in the control group(HCs).Demographic and clinical data were collected for all participants.The Movement Disorder Society-Unified Parkinson’s Disease Rating Scale(MDS-UPDRS),Hoehn and Yahr(H-Y)scale,Mini-Mental State Examination(MMSE),and Montreal Cognitive Assessment(MoCA)were used to as-sess the patients in the early-onset PD group.Electrochemiluminescence immunoassay was performed to mea-sure the levels ofα-Syn monomers and aggregates in the RBCs of both groups.The correlation between RBC-derivedα-Syn levels and clinical indicators in patients with early-onset PD was analyzed.Results:The levels of bothα-Syn monomers and aggregates in RBCs were significantly higher in patients with early-onset PD than in HCs(P=0.009,P<0.0001).In terms of diagnostic performance,RBC-derivedα-Syn aggregates showed superior performance over monomers,with an area under the receiver operating characteristic curve(AUC)of 0.887(95%CI 0.794-0.981),73.3%sensitivity,and 96.2%specificity for diagnosing early-onset PD.The levels of RBC-derivedα-Syn monomers and aggregates showed no significant correlation with patient age,disease duration,H-Y stage,MDS-UPDRS III score,MMSE score,or MoCA score(P>0.05).Conclusion:Ab-normalα-Syn deposition was found in the RBCs of patients with early-onset PD.RBC-derivedα-Syn,particular-lyα-Syn aggregates,could serve as a biomarker for early-onset PD.

关 键 词：红细胞 Α-突触核蛋白 早发型帕金森病 生物标记物 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学] R742