An experimental research into endostatin microbubble combined with focused ultrasound for anti-tumor angiogenesis in colon cancer  

作　　者：Xiufeng Zhang Guangen Yang Ying Zhang Pintong Huang Jianming Qiu Yu Sun Zhong Shen Xiujun Liao Hongsheng Xia Shuxian Shao Dong Wang 

机构地区：[1]Department of Colorectal Surgery,The Third People’s Hospital of Hangzhou,Hangzhou City,Zhejiang,China [2]Department of Ultrasonography,The Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou City,Zhejiang,China [3]The Original Biological Science and Technology Co.Ltd.,Nanjing City,Jiangsu,China [4]Department of Ultrasonography,The Third People’s Hospital of Hangzhou,Hangzhou City,Zhejiang,China

出　　处：《Gastroenterology Report》2014年第1期44-53,共10页胃肠病学报道（英文）

基　　金：This work was supported by the following grants:National Natural Science Foundation of China(No.81071164,No.81271584);Zhejiang Provincial Natural Science Foundation of China(No.Y2110782);Public Technology for Applied Research Program Foundation from the Science and Technology Bureau of Zhejiang Province(No.2013C31002);Key Program Foundation from the Health Bureau of Zhejiang Province(No.2013ZDA020);the General Program Foundation from the Hangzhou City Health Bureau(No.2013A27).

摘　　要：Objective:to evaluate the therapeutic effect of targeted endostatin-loaded microbubbles,combined with improved,focused,directional ultrasound radiation for inhibition of subcutaneous translocation in situ colon tumor angiogenesis in colon cancer.Methods:65 BALB/c nude mice with subcutaneous translocation in situ colon tumors were randomly divided into five groups.Group A was the control group,without any treatments.In Group B,the mouse was treated with ultrasonic radiation.In Group C,the mouse was treated with ultrasonic radiation combined with empty SonoVue microbubbles.In Group D,the mouse was treated with ultrasonic radiation combined with empty Targestar-SA microbubbles.In Group E,the mouse was treated with ultrasonic radiation combined with endostatin microbubbles.The tumor size was measured before and 1,14,and 28 days after irradiation.The peak intensity(PI),regional blood volume(RBV)and regional blood flow(RBF)were recorded using contrast-enhanced ultrasound.The tumor tissue was removed for pathological examination;the tumor necrosis area and microvascular density(MVD)were evaluated by immunohistochemistry.Results:Tumors in Groups C,D and E were significantly smaller than in Groups A and B at 28 days after irradiation,with Group E the smallest.PI,RBF and RBV of Groups C,D,and E were significantly decreased 28 days after radiation with Group E the lowest,and significantly lower than Groups A and B(all P<0.05).The tumor tissue necrosis area of Group E was clearly greater while MVD was obviously lower than the other groups(all P<0.01)at 28 days after treatment.Conclusion:The targeted endostatin microbubbles,combined with focused,directional ultrasound radiation can damage tumor microvasculature of subcutaneous colon translocation in situ colon cancer,as well as inhibit the tumor angiogenesis.

关 键 词：colorectal cancer tumor angiogenesis ultrasonic cavitation ENDOSTATIN 

分 类 号：R73[医药卫生—肿瘤]