Effects of Wumen Gumi Bao Decoction on Ameliorating Bone Loss in Experimentally Induced Osteoporosis in Rats by Regulating Wnt3a/β-Catenin Pathway  

作　　者：Guodong ZHANG Yongqing HUA Qihan MA Guoqiang LIANG 

机构地区：[1]Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou 215009,China [2]Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China

出　　处：《Medicinal Plant》2023年第3期57-60,65,共5页药用植物：英文版

基　　金：Supported by Senile Health Research Project of Jiangsu Provincial Health Commission of China(LKZ2023217);Natural Science Foundation of Nanjing University of Traditional Chinese Medicine(XAR2021041);Suzhou Science and Technology Development Plan Project(SYSD2020215,SKY2022202);The Ninth Batch of Suzhou Gusu Health Key Talents Project(GSWS2022107)。

摘　　要：[Objectives]To investigate the preventive effects of Wumen Gumi Bao Decoction(WMGBD)on estrogen deficiency-induced bone loss.[Methods]Three-month-old Sprague-Dawley rats were ovariectomized(OVX)and then treated with WMGBD,and their admixtures for six weeks.The bone trabecular microstructure,bone histopathological examination were determined in the rat femur tissue,and serum biomarkers of bone formation and resorption were analyzed by ELISA,and the protein expressions of Wnt3a,β-catenin,and phosphorylatedβ-catenin(p-β-catenin)were analyzed by Western blot.Statistical analysis was conducted by using one-way analysis of variance(ANOVA)followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0.[Results]WMGBD could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats.Furthermore,WMGBD also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue,while WMGBD could activate the Wnt3a/β-catenin pathway.[Conclusions]WMGBD could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.

关 键 词：Wumen Gumi Bao Decoction(WMGBD) OSTEOPOROSIS Bone loss Wnt3a/β-catenin pathway 

分 类 号：R285.5[医药卫生—中药学]