一起由金黄色葡萄球菌引起的食源性疾病的微生物检测与分析  

Microbial detection and analysis of a foodborne disease caused by Staphylococcus aureus infection

作　　者：陈少华[1] 刘了了 彭康 Chen Shaohua;Liu Liaoliao;Peng Kang(Department of Microbiological Testing,Hefei Center for Disease Control and Prevention,Hefei 230091,Anhui Province,China)

机构地区：[1]合肥市疾病预防控制中心微生物检验科,合肥230091

出　　处：《中国基层医药》2023年第5期741-746,共6页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的利用多种检测方法查明一起疑似细菌性食物中毒引起的食源性疾病的病因。方法2022年8月9日上午9时35分合肥市疾病预防控制中心处理的一起疑似食源性疾病事件,实验室检测结果初步显示为金黄色葡萄球菌(金葡菌)感染。运用实时荧光聚合酶链式反应(RT-PCR)方法检测分离培养鉴定所得的16株金葡菌的肠毒素基因(SEA-SEE)。同时对分离出的16株不同来源的金葡菌菌株运用脉冲场凝胶电泳(PFGE)技术进行分子分型,分析菌株之间的相关性。结果分离到的16株金葡菌肠毒素(SEs)分布情况是李姓售卖员肛拭子和砧板(内)涂抹样为SEB型,其余14株都同时携带A、C、E型肠毒素基因。PFGE分型结果显示所有菌株分为2个型别,除李姓售卖员肛拭子和砧板(内)涂抹样外其他14株均为同一型别,相似度为100%。李姓售卖株员肛拭子和砧板(内)涂抹样相似度为80.65%,与另一型别相似度为59.26%。这一结果和SEs的检测结果基本一致。结论该例食源性疾病是由具有肠毒素的2种或3种不同来源的金葡菌混合污染引起的。Objective To identify the cause of a suspected foodborne disease caused by bacterial food poisoning using multiple detection methods.Methods At 9:35 a.m.on August 9,2022,a suspected foodborne disease incident was handled by the Hefei Center for Disease Control and Prevention.The preliminary laboratory test results showed that the foodborne disease was caused by Staphylococcus aureus infection.Sixteen strains of Staphylococcus aureus were isolated,cultured,and identified using real-time fluorescent polymerase chain reaction.At the same time,the 16 strains of Staphylococcus aureus isolated from different sources were analyzed by pulsed-field gel electrophoresis to determine the correlation between strains.Results The distribution of 16 strains of Staphylococcus aureus enterotoxins isolated was that the anal swab and chopping board(inner)smear samples from a salesperson surnamed Li showed SEB type,and the remaining 14 strains all carried type A,C,and E enterotoxin genes simultaneously.Pulsed-field gel electrophoresis typing results showed that there were two types of strains;except the anal swab and chopping board(inner)smear samples from the salesperson surnamed Li,the other 14 strains were all of the same type,with a similarity of 100%.The similarity between the anal swab and the chopping board(inner)smear samples from the salesperson surnamed Li was 80.65%,and the similarity with another type was 59.26%.This result was consistent with the detection results of Staphylococcus aureus enterotoxins.Conclusion The foodborne disease in this case was caused by mixed contamination of two or three different sources of Staphylococcus aureus with enterotoxins.

关 键 词：金黄色葡萄球菌 肠毒素类 聚合酶链反应 电泳 凝胶 脉冲场 食物传播疾病 

分 类 号：R155.5[医药卫生—营养与食品卫生学]