消岩汤联合顺铂抑制肺腺癌H1975细胞生长  

Xiaoyan decoction combined with cisplatin inhibits the growth of lung adenocarcinoma H1975cells

作　　者：韩燕燕[1] 郑旭[1] 兰福 李翀[1] HAN Yanyan;ZHENG Xu;LAN Fu(Department of Pathology,First Teaching Hospital,Tianjin University of Traditional Chinese Medicine,Tianjin 300000,CHINA)

机构地区：[1]天津中医药大学第一附属医院病理科(国家中医针灸临床医学研究中心),300000

出　　处：《江苏医药》2023年第5期433-437,F0002,共6页Jiangsu Medical Journal

基　　金：国家自然科学基金(81774054);天津中医药大学第一附属医院“拓新工程”基金科研课题(院YB202111)。

摘　　要：目的探讨消岩汤联合顺铂对肺腺癌H1975细胞增殖、DNA损伤及凋亡的影响。方法采用CCK-8实验检测不同浓度消岩汤及消岩汤与不同浓度顺铂联合用药对肺腺癌H1975细胞增殖的作用,筛选最佳作用浓度。采用细胞克隆形成实验、细胞免疫荧光和DNA修复实验分别检测空白对照组、消岩汤10mg/mL组、顺铂25μmol/L组和联合用药组(消岩汤10mg/mL+顺铂25μmol/L)的细胞增殖、DNA损伤和修复能力。实时荧光定量PCR检测各组细胞凋亡相关因子Bcl-2、Bax和Caspase-3mRNA表达。结果与消岩汤10mg/mL组和顺铂25μmol/L组相比,联合用药组可抑制H1975细胞增殖活性及克隆形成,诱导DNA损伤,抑制Bcl-2mRNA表达和提高Bax、Caspase-3mRNA表达(P<0.05)。结论消岩汤联合顺铂可抑制肺腺癌H1975细胞的生长,其机制可能与抑制细胞增殖、调控细胞DNA损伤和修复及介导Bcl-2、Bax和Caspase-3表达有关。Objective To investigate the effects of Xiaoyan decoction combined with cisplatin on the proliferation,DNA damage,and apoptosis of lung adenocarcinoma H1975cells.Methods CCK-8 experiment was used to evaluate the effects of gradient concentrations of Xiaoyan decoction,and Xiaoyan decoction combined with gradient concentrations of cisplatin on the proliferation of lung adenocarcinoma H1975cells,and to screen the optimal concentration of action.The cell clone formation test,cellular immunofluorescence test,and DNA repair test were used to detect the proliferation,DNA damage and repair ability in the blank control group,Xiaoyan decoction 10 mg/mL group,cisplatin 25μmol/L group,and combined medication group(Xiaoyan decoction 10mg/mL+cisplatin 25μmol/L).The expressions of apoptosis-related factors Bcl-2,Bax,and Caspase-3mRNA in each group were detected by real-time fluorescence quantitative PCR.Results Compared with Xiaoyan decoction 10mg/mL group and cisplatin 25μmol/L group,the combined medication group showed an inhibition of the proliferation activity and clone formation of H1975cells,with induced DNA damage,inhibited mRNA expression of Bcl-2,and increased mRNA expressions of Bax and Caspase-3(P<0.05).Conclusion Xiaoyan decoction combined with cisplatin can inhibit the growth of lung adenocarcinoma H1975cells and the mechanism for it may be related to inhibiting cell proliferation,regulating cell DNA damage and repair,and mediating the expressions of Bcl-2,Bax and Caspase-3.

关 键 词：消岩汤 顺铂 肺腺癌细胞 增殖 脱氧核糖核酸损伤 凋亡 

分 类 号：R734[医药卫生—肿瘤]