G15逆转六味地黄汤冻干粉对慢性应激海马细胞的保护作用  

G15 Reversing Protective Effect of Liuwei Dihuang Decoction(六味地黄汤)Lyophilized Powder on Hippocampal Cells under Chronic Stress

作　　者：徐碧云[1,2,3] 薛岱榆 杨志敏 XU Biyun;XUE Daiyu;YANG Zhimin(The Second Clinical School of Guangzhou University of Chinese Medicine,Guangzhou 510120,Guangdong,China;Guangzhou University of Chinese Medicine,Guangzhou 510720,Guangdong,China;Zhuhai Center for Chronic Disease Control,Zhuhai 519000,Guangdong,China;不详)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510120 [2]广州中医药大学,广东广州510720 [3]珠海市慢性病防治中心,广东珠海519000 [4]不详

出　　处：《辽宁中医杂志》2023年第4期142-145,I0003,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81603551);杨志敏广东省名中医传承工作室建设项目(粤中医办函(2020)1号)。

摘　　要：目的探讨六味地黄汤(LW)冻干粉对皮质酮诱导损伤的HT22海马细胞的保护作用及G15的逆转作用。方法利用皮质酮构建慢性应激损伤HT22神经元细胞体外模型,将神经元分为对照组,模型组,LW(320 mg·L^(-1))组、LW+G15低剂量组(10μmol·L^(-1))和LW+G15高剂量组(30μmol·L^(-1)),并进行相应干预。采用四甲基偶氮唑盐(MTT)法检测HT22细胞的存活率。采用TUNNEL染色检测细胞凋亡率。结果与对照组比较,CORT诱导损伤的模型组细胞OD值显著下降和细胞凋亡数明显增加(P<0.05)。与模型组比较,LW可明显增加海马细胞OD值和降低凋亡细胞(P<0.05)。与LW组比较,LW+G15高剂量组可降低海马细胞OD值和增加凋亡细胞(P<0.05)。结论G1530μmol·L^(-1)可逆转LW冻干粉对皮质酮损伤小鼠HT22海马细胞的保护作用。Objective To investigate the protective effect of Liuwei Dihuang Decoction(六味地黄汤,LW)on corticosterone-induced HT22 hippocampal cells and the reversal effect of G15.Method In vitro model of HT22 neurons injured by chronic stress was established by using corticosterone.The neurons were divided into control group,model group,LW(320 mg·L^(-1))group,LW+G15 low-dose group(10μmol·L^(-1))and LW+G15 high-dose group(30μmol·L^(-1)),and the corresponding intervention was performed.The survival rate of HT22 cells was determined by tetramethylazolium salt(MTT)assay.The apoptosis rate was detected by TUNNEL staining.Result Compared with the normal group,the model group significantly dsecreased the OD value of hippocampal cells and increased the apoptotic cells(P<0.05).Compared with the model group,LW significantly increased the OD value of hippocampal cells and decreased the apoptotic cells(P<0.05).Compared with the LW group,LW+30μmol·L^(-1)G15 decreased the OD value of hippocampal cells and increased the apoptotic cells(P<0.05).Conclusion G1530μmol·L^(-1)could reverse the protective effect of LW lyophilized powder on corticosterone-injured mice HT22 hippocampal cells.

关 键 词：六味地黄汤 皮质酮 海马细胞 雌激素 G15 

分 类 号：R574.62[医药卫生—消化系统]