子宫内膜癌中lncRNA KRT7-AS的表达及其对HEC-1A细胞增殖的影响  

Expression of lncRNA KRT7-AS in endometrial carcinoma and its effect on the proliferation of HEC-1A cells

作　　者：张敏[1] 周华[1] 李华[1] 吴芳 张晟[1] ZHANG Min;ZHOU Hua;LI Hua;WU Fang;ZHANG Sheng(Dept.of Physiology,Anhui Medical College,Hefei 230601,China)

机构地区：[1]安徽医学高等专科学校生理学教研室,安徽合肥230601

出　　处：《华夏医学》2023年第2期31-37,共7页Acta Medicinae Sinica

基　　金：2020年度安徽省高校自然科学研究项目(KJ2020A0860)。

摘　　要：目的:探索子宫内膜癌(EC)中长链非编码RNA KRT7-AS的表达及其对HEC-1A细胞增殖的影响。方法:采用在线数据库检测分析KRT7-AS在EC及癌旁正常组织中的表达;用RT-qPCR法检测人子宫内膜癌细胞系HEC-1A、HEC-1B、KLE、B-MD-1C细胞中KRT7-AS的表达;分别构建KRT7-AS敲低(shKRT7-AS)和对照(shCtrl)慢病毒。慢病毒感染HEC-1A细胞并培养5 d后,采用RT-qPCR检测敲低效率,MTT法检测细胞增殖,流式细胞术测定细胞周期和凋亡。结果:在EC组织中,KRT7-AS表达高于癌旁组织(P<0.05),KRT7-AS高表达患者的总生存数低于低表达患者(P<0.05)。KRT7-AS敲低组的HEC-1A在第5天增殖速率显著降低(P<0.05)。KRT7-AS敲低导致HEC-1A细胞停滞于G0/G1期(P<0.05);细胞凋亡比例则显著增加(P<0.05)。结论:KRT7-AS异常高表达促进EC的发展。Objective:To explore the expression of long non-coding RNA KRT7-AS in endometrial carcinoma(EC)and its effect on the proliferation of HEC-1A cells.Methods:The online database was used to analyze the expression of KRT7-AS in EC and normal tissue adjacent to cancer;RT-qPCR was used to detect the human endometrial cancer cell lines KLE,HEC-1A,HEC-1B,B-MD-1C expression in KRT7-AS cells;KRT7-AS knockdown(shKRT7-AS)and control(shCtrl)lentiviruses were constructed,respectively.After lentivirus-infected HEC-1A cells and cultured for 5 days,the knockdown efficiency was detected by RT-qPCR,cell proliferation was detected by MTT method,and cell cycle and apoptosis were detected by flow cytometry.Results:The expression of KRT7-AS in EC tissue was significantly higher than that in adjacent tissue(P<0.05),and the overall survival rate of patients with high KRT7-AS expression was significantly lower than that of patients with low KRT7-AS expression(P<0.05).The proliferation rate of HEC-1A in the KRT7-AS knockdown group was significantly decreased on the 5th day(P<0.05).KRT7-AS knockdown caused HEC-1A cells to arrest in G0/G1 phase(P<0.05),while the proportion of apoptosis was significantly increased(P<0.05).Conclusion:The abnormally high expression of KRT7-AS may promote the development of EC.

关 键 词：长链非编码RNA KRT7-AS 细胞增殖 子宫内膜癌 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]