Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential  

作　　者：Lu Li Pishun Li Jiale Chen Li Li Yunfan Shen Yangzixuan Zhu Jiayi Liu Lu Lv Song Mao Fang Chen Guang Hu Kai Yuan 

机构地区：[1]Hunan Key Laboratory of Molecular Precision Medicine,Department of Oncology,Xiangya Hospital,Central South University,Changsha,Hunan,China [2]Hunan Key Laboratory of Medical Genetics,School of Life Sciences,Central South University,Changsha,Hunan,China [3]Changjun High School,Changsha,Hunan,China [4]Zhounan High School,Changsha,Hunan,China [5]Epigenetics and Stem Cell Biology Laboratory,National Institute of Environ-mental Health Sciences,Research Triangle Park,NC 2779,USA [6]National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha,Hunan,China [7]The Biobank of Xiangya Hospital,Central South University,Changsha,Hunan,China

出　　处：《Cell Regeneration》2022年第1期246-266,共21页细胞再生（英文）

基　　金：This project has been supported by the National Natural Science Foundation of China(32170821,31771589 to K.Y);Ministry of Science and Technology of the People’s Republic of China(2021YFC2701202);Department of Science&Technology of Hunan Province(2021JJ10054,2019SK1012,2018DK2015,2017RS3013,2017XK2011 to K.Y,2019JJ40478 to P.L,and the innovative team program 2019RS1010);Central South University(2018CX032 to K.Y,and the innovation-driven team project 2020CX016)。

摘　　要：Mouse embryonic stem cells(mESCs)cycle in and out of a transient 2-cell(2C)-like totipotent state,driven by a com-plex genetic circuit involves both the coding and repetitive sections of the genome.While a vast array of regulators,including the multi-functional protein Rif1,has been reported to influence the switch of fate potential,how they act in concert to achieve this cellular plasticity remains elusive.Here,by modularizing the known totipotency regulatory factors,we identify an unprecedented functional connection between Rif1 and the non-canonical polycomb repres-sive complex PRC1.6.Downregulation of the expression of either Rif1 or PRC1.6 subunits imposes similar impacts on the transcriptome of mESCs.The LacO-LacI induced ectopic colocalization assay detects a specific interaction between Rif1 and Pcgf6,bolstering the intactness of the PRC1.6 complex.Chromatin immunoprecipitation followed by sequencing(ChIP-seq)analysis further reveals that Rif1 is required for the accurate targeting of Pcgf6 to a group of genomic loci encompassing many genes involved in the regulation of the 2C-like state.Depletion of Rif1 or Pcgf6 not only activates 2C genes such as Zscan4 and Zfp352,but also derepresses a group of the endogenous retroviral element MERVL,a key marker for totipotency.Collectively,our findings discover that Rif1 can serve as a novel auxiliary component in the PRC1.6 complex to restrain the genetic circuit underlying totipotent fate potential,shedding new mechanistic insights into its function in regulating the cellular plasticity of embryonic stem cells.

关 键 词：2C-like TOTIPOTENCY MERVL Rif1 PRC1.6 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]