Cilia regeneration requires an RNA splicing factor from the ciliary base  

作　　者：Kaiming Xu Guangshuo Ou 

机构地区：[1]Tsinghua-Peking Center for Life Sciences,Beijing Frontier Research Center for Biological Structure,McGovern Institute for Brain Research,School of Life Sciences and MOE Key Laboratory for Protein Science,Tsinghua University,Beijing,China

出　　处：《Cell Regeneration》2022年第1期303-314,共12页细胞再生（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(grants 31991190,31730052,31525015,31861143042,31561130153,31671444,31871352);the National Key R&D Program of China(2017YFA0503501,2019YFA0508401,and 2017YFA0102900)。

摘　　要：Cilia are microtubule-based organelles projected from most eukaryotic cell surfaces performing cell motility and signaling.Several previously recognized non-ciliary proteins play crucial roles in cilium formation and function.Here,we provide additional evidence that the Caenorhabditis elegans RNA splicing factor PRP-8/PRPF8 regulates ciliogen-esis and regeneration from the ciliary base.Live imaging of GFP knock-in animals reveals that the endogenous PRP-8 localizes in the nuclei and the ciliary base.A weak loss-of-function allele of prp-8 affects ciliary structure but with little impact on RNA splicing.Conditional degradation of PRP-8 within ciliated sensory neurons showed its direct and spe-cific roles in cilium formation.Notably,the penetrance of ciliary defects correlates with the reduction of PRP-8 at the ciliary base but not nuclei,and sensory neurons regenerated cilia accompanying PRP-8 recovery from the ciliary base rather than the nuclei.We suggest that PRP-8 at the ciliary base contributes to cilium formation and regeneration.

关 键 词：PRP-8/PRPF8 RNA splicing factor CILIOGENESIS Cilium regeneration 

分 类 号：Q24[生物学—细胞生物学]