Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism  

作　　者：Ning Huang Zu-Hang Sheng 

机构地区：[1]Synaptic Function Section,The Porter Neuroscience Research Center,National Institute of Neurological Disorders and Stroke,National Institutes of Health,Room 2B-215,35 Convent Drive,Bethesda,MD 20892-3706,USA [2]Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Xi’an Jiaotong University Health Science Center,Xi’an 710061,Shaanxi,China [3]Institute of Neuroscience,Translational Medicine Institute,Xi’an Jiaotong University Health Science Center,Xi’an 710061,Shaanxi,China

出　　处：《Cell Regeneration》2022年第1期337-347,共11页细胞再生（英文）

基　　金：This work was supported by grants from the“Young Talent Support Plan”of Xi’an Jiaotong University(71211222010704)to N.Huang;the Intramural Research Program of NINDS,NIH(ZIA NS003029 and ZIA NS002946)to Z.-H.Sheng.

摘　　要：Central nervous system(CNS)neurons typically fail to regenerate their axons after injury leading to neurological impairment.Axonal regeneration is a highly energy-demanding cellular program that requires local mitochondria to supply most energy within injured axons.Recent emerging lines of evidence have started to reveal that injury-triggered acute mitochondrial damage and local energy crisis contribute to the intrinsic energetic restriction that accounts for axon regeneration failure in the CNS.Characterizing and reprogramming bioenergetic signaling and mitochondrial maintenance after axon injury-ischemia is fundamental for developing therapeutic strategies that can restore local energy metabolism and thus facilitate axon regeneration.Therefore,establishing reliable and reproduc-ible neuronal model platforms is critical for assessing axonal energetic metabolism and regeneration capacity after injury-ischemia.In this focused methodology article,we discuss recent advances in applying cutting-edge microflu-idic chamber devices in combination with state-of-the-art live-neuron imaging tools to monitor axonal regeneration,mitochondrial transport,bioenergetic metabolism,and local protein synthesis in response to injury-ischemic stress in mature CNS neurons.

关 键 词：Microfluidic device Axon injury ISCHEMIA Axon regeneration Mitochondrial transport Axonal bioenergetics Axonal protein synthesis 

分 类 号：R74[医药卫生—神经病学与精神病学]