大鼠胃十二指肠动脉逆行给药的研究  

Retrograde administration of drugs via gastroduodenal artery in rats:an experimental study

作　　者：赵航 郭思敏 马中翔 张昊 陈妙玲 武春雪 韩建军[1] ZHAO Hang;GUO Simin;MA Zhongxiang;ZHANG Hao;CHEN Miaoling;WU Chunxue;HAN Jianjun(Department of Intervention,Shandong Provincial Cancer Hospital,Jinan,Shandong Province 250000,China)

机构地区：[1]山东省肿瘤医院介入科,山东济南250000

出　　处：《介入放射学杂志》2023年第5期456-458,共3页Journal of Interventional Radiology

基　　金：国家重点研发计划(2018YFE0126500);山东省自然科学基金(ZR2021MH060)。

摘　　要：目的 比较大鼠胃十二指肠动脉逆行给药的新方法和传统方法的优缺点。方法 30只Wistar大鼠随机分为A、B两组,每组各15只。A组用新方法,B组用传统方法 。比较两组大鼠手术成功率、手术用时、术中意外、大鼠术后恢复及7 d生存情况。结果 A组成功14只,用时(22.8±0.51) min,其中1只因在注射药物时手抖针头扯破血管致给药失败。B组成功8只,用时(32.5±0.55) min,其中5只因胃十二指肠动脉过于纤细,无法将血管准确的剪开1/3~1/2致插管失败;另外2只因针鞘滑出血管致实验失败。A组14只术后10 min左右苏醒,1~2 h开始活动,8~10 h开始进食饮水,24~36 h基本与正常大鼠无异。B组8只术后10 min左右苏醒,3 h左右开始活动,12 h左右开始进食饮水,36 h后状态接近正常大鼠,但7 d内进食饮水量明显不及A组大鼠,两组手术成功的大鼠7 d内皆存活。结论 新方法操作简单,成功率高,可重复性强。Objective To compare the advantages and disadvantages between the retrograde administration of drugs via gastroduodenal artery(a new method of drug infusion)and the conventional method.Methods A total of 30 Wistar rats were randomly and equally divided into group A(n=30)and group B(n=30).The new method was employed in group A,while the conventional method was used in group B.The surgical success rate,time spent for operation,intraoperative accident,postoperative recovery,and 7-day survival were compared between the two groups.Results In group A,successful drug infusion was accomplished in 14 rats,and the mean time spent for operation was(22.8±0.51)min.In one rat,the injection of drug failed because the needle tore the blood vessel due to the operator's hand trembling.In group B,successful drug infusion was accomplished in 8 rats,and the mean time spent for operation was(32.5±0.55)min.In 5 rats the catheterization failed because the gastroduodenal artery was too thin to be cut by its 1/3 to 1/2 diameter,and in other 2 rats the drug infusion failed as the needle sheath slipped out of the blood vessel.In group A,14 rats woke up about 10 min after surgery,started moving within 1-2 h after surgery,started eating and drinking within 8-10 h after surgery,and became basically no different from normal rats within 24-36 h after surgery.In group B,8 rats woke up about 10 min after surgery,started moving about 3 h after surgery,started eating and drinking about 12 h after surgery,and became basically no different from normal rats about 36 h after surgery,but the intake of water within 7 d was significantly less than that of the rats in group A.All the rats having successful operation in both groups survived within 7 days.Conclusion The retrograde administration of drugs via gastroduodenal artery is a new method of drug infusion,it is simple to operate with high success rate and high repeatability.

关 键 词：胃十二指肠动脉逆行给药技术 动物实验 介入放射学 大鼠 肝动脉化疗栓塞术 

分 类 号：R730.5[医药卫生—肿瘤]