DNA/RNA helicase DHX36 is required for late stages of spermatogenesis  

作　　者：Kejia Zhang Tianxin Zhang Yujie Zhang Jinyu Yuan Xinzhe Tang Chaobao Zhang Qianqian Yin Yonglian Zhang Ming-Han Tong 

机构地区：[1]School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Hangzhou 310024,China [2]State Key Laboratory of Molecular Biology,Shanghai Key Laboratory of Molecular Andrology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Journal of Molecular Cell Biology》2022年第11期50-61,共12页分子细胞生物学报（英文版）

基　　金：supported by grants from the National Key R&D Program of China(2021YFC2700200 and 2022YFC2702602);the National Natural Science Foundation of China(31930034);the Science and Technology Commission of Shanghai Municipality(19JC1415800 to M.-H.T.).

摘　　要：Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.

关 键 词：Dhx36 MEIOSIS guanine-quadruplex(G4) Spo11 SYNAPSIS meiotic recombination crossover 

分 类 号：Q943.2[生物学—植物学]