酵母和脂多糖诱导小鼠不同的发热反应  

Yeast and lipopolysaccharide induce different febrile responses in mice

作　　者：梁艺潇 许阳 占汇东 王静[1,2] 吴珊珊[1,2] 葛悦萍[1,2] 彭力 管庆波 何钊[1,2] LIANG Yixiao;XU Yang;ZHAN Huidong;WANG Jing;WU Shanshan;GE Yueping;PENG Li;GUAN Qingbo;HE Zhao(Shandong University,Ji'nan 250012,China;Department of Endocrinology,Shandong Provincial Hospital,Shandong Key Laboratory of Endocrinology and Lipid Metabolism,Shandong Institute of Endocrine and Metabolic Diseases,Shandong Engineering Laboratory of Prevention and Control for Endocrine and Metabolic Diseases,Ji'nan 250021,China)

机构地区：[1]山东大学,济南250012 [2]山东省立医院内分泌科,山东省内分泌与脂代谢重点实验室,山东省内分泌与代谢病研究所,山东省内分泌与代谢性疾病防治工程实验室,济南250021

出　　处：《生命的化学》2023年第4期610-621,共12页Chemistry of Life

摘　　要：真菌感染致持久反复的低热,而细菌感染致高热或低体温,但这种差异性发热机理不清。本文使用酵母和脂多糖(LPS)建立小鼠发热模型,用不同测温方法(直肠测温、红外测温、腹部遥测)和在不同温度(30℃、26℃和22℃)下分别比较酵母和脂多糖致热效果及下丘脑视前区(POA)神经元活性。结果发现,酵母诱导发热不被环境温度变化和操作刺激影响;酵母和LPS分别激活了不同的POA神经元,酵母和LPS诱导的发热与腹内侧视前区(VMPO)相关,而内侧视前区(MPO)与LPS诱导低体温相关。本研究结果为阐明真菌和细菌诱导不同发热反应机制研究提供了基础。Fungal infection is always associated with prolonged and low-grade fever,while bacterial infection results in either fever or hypothermia.However,the cause of differential pyrogenic manifestations between fungi and bacteria remains elusive.In this study,yeast and lipopolysaccharide(LPS)were used to establish fever models in mice,the pyrogenetic activity between baker yeast and lipopolysaccharide,and the activation of the preoptic area of hypothalamus(POA)neurons are observed via different temperature measurement approaches(rectal thermometer,infrared thermometer,abdominal telemetry)and different ambient temperatures(30℃,26℃,22℃).The results show that yeast infection produces a robust febrile response independent of changes in ambient temperature and insensitive to experimental stimuli;yeast and LPS activate different POA neurons respectively,yeast and LPS-induced fever are associated with the ventromedial preoptic area(VMPO),while the medial preoptic area(MPO)is associated with LPS-induced hypothermia.These results provide a preliminary basis for further elucidating the mechanism of different febrile reactions induced by fungi and bacteria.

关 键 词：细菌性发热 真菌性发热 脂多糖 酵母 环境温度 下丘脑视前区 红外测温法 

分 类 号：R441.3[医药卫生—诊断学]