毛果杨基因家族的密码子特性分析  

Codon Usage Analysis of Gene Families in Populus tomentosa

作　　者：李健[1,2] 刘家福 徐宏蔚 刘晓 王艳 徐吉臣 Li Jian;Liu Jiafu;Xu Hongwei;Liu Xiao;Wang Yan;Xu Jichen(National Engineering Laboratory of Tree Breeding,Beijing Forestry University,Beijing,100083;Shandong Institute of Pomology,Taian,271000)

机构地区：[1]北京林业大学,林木育种国家工程实验室,北京100083 [2]山东省果树研究所,泰安271000

出　　处：《分子植物育种》2023年第12期3898-3906,共9页Molecular Plant Breeding

基　　金：北京林业大学大学生科研训练计划项目资助。

摘　　要：本研究拟探讨杨树基因家族的密码子特征,为深入解析杨树基因家族的进化规律并为分子育种奠定基础。本研究基于测序的毛果杨基因组,鉴定扩展蛋白(Expansin)、肌动蛋白(Actin)、纤维素合酶(Cellulose synthase,Ces)、果胶甲酯酶(pectin methylesterase,Pmes)和木葡聚糖内糖基转移酶/水解酶(xyloglucan endotransglucosylase/hydrolase,Xth)5个基因家族序列,并利用CodonW等软件分析5个家族基因密码子的碱基组成特征。结果显示,毛果杨5个基因家族密码子的GC含量、有效密码子数(ENC)相似,密码子第三位碱基都偏好使用碱基T,具有一定的物种特征。不同基因家族也存在一定程度的特异性,分别含有9(Actin)、9(Ces)、5(Pmes)、6(Expansin)、4(Xth)个高频密码子,以及22(Actin)、9(Ces)、14(Pmes)、19(Expansin)、16(Xth)个最优密码子。毛果杨基因密码子使用偏性较弱,不同基因家族进化的方向和速率存在一定的差异。The study aims to explore the codon usage pattern of the poplar gene families,so as to reveal the gene evolutionary rules of the poplar gene families and lay a foundation for the future molecular breeding.Based on the sequencing genome data of Populus tomentosa,the gene sequences of the five gene families,Expansin,Actin,Pectin methylesterase(Pmes),Cellulose synthase(Ces),and Xyloglucan endotransglucosylase/hydrolase(Xth)were identified.The base compositions were individually evaluated with CodonW software.The codon usage patterns in different gene families of Populus trichocarpa were highly similar and displayed species characteristically,such as GC content,ENC value,and the third base of codon prefer to use base T.Meanwhile,certain changes were present among the gene families.Each family individually contains 9(Actin),9(Ces),5(Pmes),6(Expansin),and 4(Xth)high frequency codons,while each family contains 22(Actin),9(Ces),14(Pmes),19(Expansin),and 16(Xth)optimal codons,respectively.The codon usage bias of Populus tomentosa showed a lttle weak.Each gene family might evolution differently in direction and rate certainly.

关 键 词：毛果杨 基因家族 密码子偏性 基因表达 

分 类 号：S792.11[农业科学—林木遗传育种]