Regeneration and Agrobacterium-mediated genetic transformation of twelve Eucalyptus species  

作　　者：Tingting Zhou Ying Lin Yan Lin Jianzhong Luo Jihua Ding 

机构地区：[1]College of Horticulture and Forestry,Hubei Hongshan Laboratory,Hubei Engineering Technology Research Center for Forestry Information,Huazhong Agricultural University,Wuhan 430070,China [2]Research Institute of Fast-growing Trees(RIFT)/China Eucalypt Research Centre(CERC),Chinese Academy of Forestry(CAF),ZhanJiang,GuangDong,China

出　　处：《Forestry Research》2022年第1期149-156,共8页林学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31971676);the Opening Project of State Key Laboratory of Tree Genetics and Breeding(K2020103);the Fundamental Research Funds for Central Universities(2662019PY007)。

摘　　要：Eucalyptus is a genus of over 900 species and hybrids,and many of them are valuable fast-growing hardwoods.Due to its economic importance,Eucalyptus is one of the early tree species whose genomes were deciphered.However,the lack of efficient genetic transformation systems severely restricts the functional genomic research on the plant.The success of Eucalyptus regeneration and transformation depends greatly on the genotypes and explants.In this study,we systematically screened 26 genotypes from 12 Eucalyptus species in an attempt to obtain Eucalyptus genotypes with high regeneration potential.We developed two common regeneration media that can be applied to most tested Eucalyptus genotypes for both seeding hypocotyls and cloned internodes as explants.We then implemented DsRed2 as a visual marker for genetic transformation efficiency test.Our results suggest that E.camaldulen and E.robusta are amenable for genetic transformation.Finally,we successfully set up a stable Agrobacterium-mediated genetic transformation procedure for both E.camaldulen and E.robusta using seeding hypocotyls and cloned internodes respectively.Taken together,our study provides valuable means for vegetative propagation,gene transformation,CRISPR based gene mutagenesis,activation and suppression,as well as functional characterization of genes in Eucalyptus.

关 键 词：REGENERATION transformation ACTIVATION 

分 类 号：Q78[生物学—分子生物学]