Oxidative phosphorylation safeguards pluripotency via UDP-N-acetylglucosamine  

作　　者：Jiani Cao Meng Li Kun Liu Xingxing Shi Ning Sui Yuchen Yao Xiaojing Wang Shiyu Li Yuchang Tian Shaojing Tan Qian Zhao Liang Wang Xiahua Chai Lin Zhang Chong Liu Xing Li Zhijie Chang Dong Li Tongbiao Zhao 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology,Institute for Stem Cell and Regeneration,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [2]State Key Laboratory of Membrane Biology,School of Medicine,Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China [3]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China [4]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [5]Qufu Normal University,Qufu 273165,China [6]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Protein & Cell》2023年第5期376-381,共6页蛋白质与细胞（英文版）

基　　金：We thank Dr Masaru Okabe at Osaka University Japan for providing the B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb mice(mito-red);the National Institute for Basic Biology Japan for providing the B6.Cg-Tg(CAG-GFP/LC3)53Nmz/NmzRbrc mice.;the National Key R&D Program of China 2018YFA0108402;the Strategic Priority Research Program of the Chinese Academy of Sciences XDA16030302;the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine Grant No.33,and the National Natural Science Foundation of China Program(31720103907,31570995,31621004),to T.Z.and(31400831)to J.C.The raw data FASTQ files for the RNA sequencing data are deposited in NCBI GEO,GEO:GSE140712(Fig.1D,1E and S4)and ScienceDB,China National Standard GB/T 7714-2015(Fig.S8A,S8F and S8G);The metabolomic data files are deposited in ScienceDB,China National Standard GB/T 7714-2015.

摘　　要：Dear Editor,Embryonic stem cells(ESCs)have been assumed to possess immature mitochondria and to favor anaerobic glycolysis over oxidative phosphorylation(OXPHOS)for energy production.This proposition is largely based on the findings that ESCs possess globular mitochondria with blurred cristae,and the facts that ESCs have higher glycolysis activity and lower mitochondrial respiration capacity than somatic cells(Kondoh et al.,2007;Folmes et al.,2011,2012;Zhang et al.,2012;Xu et al.,2013;Ito and Suda,2014;Gu et al.,2016).However,recent studies have shown that mitochondrial autophagy and mitochondrial dynamics are pivotal for ESC self-renewal and pluripotency(Todd et al.,2010;Liu et al.,2016,2020;Wang et al.,2019;Zhong et al.,2019).These studies have raised a fundamental question:what is the contribution and functional mechanism of mitochondrial respiration in pluripotency regulation?

关 键 词：KONDO PHOSPHORYLATION raised 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]