下调lncRNA-ATB抑制骨肉瘤发生发展分子机制研究  

Molecular mechanism of down-regulated lncRNA-ATB inhibiting the occurrence and development of osteosarcoma

作　　者：邱明宪 QIU Ming-xian(Department of Orthopedics,the Fourth People′s Hospital of Hengshui City,Hebei Province,Hengshui 053000,China)

机构地区：[1]河北省衡水市第四人民医院骨科,河北衡水053000

出　　处：《河北医科大学学报》2023年第6期686-691,共6页Journal of Hebei Medical University

基　　金：衡水市市级科技计划(2020014068Z)。

摘　　要：目的旨在分析lncRNA-ATB对骨肉瘤Saos-2细胞增殖、周期S期聚集、迁移及凋亡的调控作用,并探究其对皮下骨肉瘤生长及细胞增殖影响。方法实时定量-聚合酶链式反应(real time quantity polymerase chain reaction,qRT-PCR)检测lncRNA-ATB在骨肉瘤组织HOS,Saos-2和U2OS细胞株及正常人成骨细胞株NHOst中的表达;CCK8、流式细胞法、Tunel及Transwell检测lncRNA-ATB对Saos-2细胞生物学功能调控能力;Ki67检测细胞增殖情况;利用Saos-2细胞构建骨肉瘤裸鼠移植瘤模型,检测裸鼠模型中皮下瘤体重量变化。结果28例患者组织中,骨肉瘤组织中的lncRNA-ATB表达水平高于癌旁正常组织(P<0.05)。与NHOst组相比,lncRNA-ATB在HOS,Saos-2和U2OS中均升高,其中Saos-2中表达最高(P<0.05)。ATB组Saos-2细胞的OD值高于NC组,ATB组Saos-2周期S期长于NC组(P<0.05)。siATB组Saos-2细胞OD值低于siNC组;siATB组Saos-2周期S期短于siNC组(P<0.05)。ATB组能抑制Saos-2细胞凋亡率,而siATB组后则相反增加Saos-2细胞的凋亡率,ATB组的细胞穿孔数多于NC组,siATB组少于siNC组(P<0.05)。ATB组皮下肿瘤重量重于NC组(P<0.05),siATB组皮下肿瘤重量轻于siNC组(P<0.05)。ATB组下骨肉瘤细胞核中阳性染色细胞百分比多于NC组,siATB组下骨肉瘤细胞核中阳性染色细胞百分比少于siNC组(P<0.05)。结论lncRNA-ATB可以促进骨肉瘤的发生发展及恶性生物学功能,而利用RNA干扰技术,干扰lncRNA-ATB抑制骨肉瘤的病理进程。Objective To investigate the regulation of lncRNA-ATB on the proliferation,aggregation,migration and apoptosis of S-phase cell cycle of osteosarcoma Saos-2 cells,and to explore its effect on the growth and cell proliferation of subcutaneous osteosarcoma.Methods Real-time quantity polymerase chain reaction(qRT-PCR)was used to detect the expression of lncRNA-ATB in osteosarcoma tissues,HOS,Saos-2 and U2OS cell lines and human osteoblast cell line NHOst.CCK8,flow cytometry,TUNEL and Transwell were used to detect the regulation of biological function of lncRNA-ATB in SAOS-2 cells.Ki67 was used to detect cell proliferation.The nude mouse xenograft model of osteosarcoma was constructed by using SAOS-2 cells,and the weight of subcutaneous tumor in nude mouse model was detected.Results In 28 patient tissues,the expression level of lncRNA-ATB in osteosarcoma tissue was higher than that in normal adjacent tissue(P<0.05).Compared with the NHOst group,lncRNA-ATB was elevated in HOS,Saos-2,and U2OS,with the highest expression in Saos-2(P<0.05).The OD value of Saos-2 cells in the ATB group was higher than that in the NC group,and the S phase of the Saos-2 cycle in the ATB group was longer than that in the NC group(P<0.05).The OD value of Saos-2 cells in the siATB group was lower than that in the siNC group,and the S phase of the Saos-2 cycle in the siATB group was shorter than that in the siNC group(P<0.05).In the ATB group,the apoptosis rate of Saos-2 cells was inhibited,while in the siATB group,the apoptosis rate of Saos-2 cells was inceased.The number of cell perforations in the ATB group was higher than that in the NC group,which was lower in the siATB group than in the siNC group(P<0.05).The weight of subcutaneous tumors was greater in the ATB group than in the NC group(P<0.05),but smaller in the siATB group than in the siNC group(P<0.05).The percentage of positive staining cells in the nucleus of osteosarcoma was higher in the ATB group than in the NC group,but lower in the siATB group than in the siNC group(P<0.05).Conc

关 键 词：骨肉瘤 lncRNA-ATB SIRNA SAOS-2 

分 类 号：R738.1[医药卫生—肿瘤]