肝清宁调控肝癌MHCC97细胞及肿瘤恶化的影响  

Experimental study on the effect of Ganqingning on biological functions of MHCC97 cells and liver cancer aggravation

作　　者：汤晓青 郭玺 万焱 王洁 徐斌 陈瑾 张洁 付海艳 罗煜 TANG Xiaoqing;GUO Xi;WAN Yan(Department of Oncology,Kunming Third People’s Hospital,Yunnan,Kunming 650000,China)

机构地区：[1]云南省昆明市第三人民医院肿瘤科,065000 [2]云南省昆明市第三人民医院医务科,065000

出　　处：《河北医药》2023年第11期1650-1653,共4页Hebei Medical Journal

基　　金：云南省科技厅科技计划项目(编号:202001BA070001-0113)。

摘　　要：目的探讨中药肝清宁对MHCC97细胞增殖、凋亡、迁移侵袭及肿瘤恶化的影响。方法不同浓度肝清宁(20、40、80μg/ml)处理MHCC97细胞,灌胃(55.36、27.68、13.84 g生药/kg)异种移植肿瘤裸鼠。对照组为正常MHCC97细胞或灌胃0.9%氯化钠溶液的荷瘤裸鼠。CCK8法、克隆形成实验检测细胞的增殖能力;流式细胞术检测细胞的凋亡能力;Transwell实验、划痕实验检测细胞的迁移侵袭能力;裸鼠成瘤实验检测肿瘤的生长情况。结果与对照组相比,肝清宁(低、中、高)浓度组细胞在24、48、72 h的存活率均显著降低,细胞的克隆形成量、迁移侵袭数和划痕愈合率均显著降低,细胞凋亡率显著升高,Cleaved caspase-3和Cleaved PARP的蛋白表达量均显著升高,MMP-3、MMP-9蛋白表达均显著降低,肿瘤的重量和体积均显著降低(P<0.05);与肝清宁低浓度组比较,肝清宁中浓度组、肝清宁高浓度组细胞的上述指标发生相同的变化(P<0.05)。结论中药肝清宁可抑制肝癌细胞增殖、迁移侵袭,促进凋亡,并抑制裸鼠体内肿瘤的生长。Objective To investigate the effects of Ganqingning on the proliferation,apoptosis,migration and invasion of MHCC97 cells and liver cancer aggravation.Methods MHCC97 cells were treated with different concentrations of Ganqingning(20,40,and 80μg/ml)and xenotransplanted tumor nude mice were gavaged with 55.36,27.68,and 13.84g crude drug/kg.MHCC97 cells treated with blank control or tumor bearing nude mice fed with 0.9%sodium chloride solution were considered as controls.Cell proliferation was detected by Cell Counting Kit-8(CCK-8)assay and clone formation assay.The apoptotic ability of cells was detected by flow cytometry.Cell migration and invasion were tested by Transwell assay and scratch test,respectively.In vivo tumor growth in nude mice was detected.Results Compared with those of the control group,the survival rate at 24,48 and 72h,number of cell clones,migratory and invasive cell numbers and wound healing rate of cells treated with low,medium and high concentrations of Ganqingning were significantly reduced,the apoptotic rate significantly increased,cleaved caspase-3 and cleaved poly(ADP-ribose)polymerase(PARP)were significantly upregulated,and matrix metalloproteinase-3(MMP-3)and MMP-9 were significantly downregulated(P<0.05).Xenotransplanted liver cancer weight and volume in mice treated with low,medium and high concentrations of Ganqingning were significantly reduced(P<0.05).Compared with the low concentration group,the above indexes of cells in the medium and high concentration groups showed the same changes(P<0.05).Conclusion Ganqingning can inhibit the proliferation,migration and invasion of liver cancer cells,promote apoptosis,and inhibit the growth of tumor in nude mice.

关 键 词：中药肝清宁 肝癌 肿瘤恶化 细胞表型 

分 类 号：R735.7[医药卫生—肿瘤]