侵袭性垂体腺瘤中TNF-αIL-6及VEGF之间表达水平变化的相关性研究  

Correlation Study of the Changes in Expression Levels between TNF-αIL-6 and VEGF in Invasive Pituitary Adenoma

作　　者：刘思洋[1] 吴建龙[1] 梅赞 苏彬[1] 王学文[1] 齐迎松 李斌斌[2] 王婷婷 张金利 高继刚 LIU Siyang;WU Jianlong;MEI Zan(Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院,河北承德067000 [2]承德护理职业学院,河北承德067000

出　　处：《河北医学》2023年第6期933-937,共5页Hebei Medicine

基　　金：2020年承德市科学技术研究与发展计划,(编号:202006A157)。

摘　　要：目的:探讨肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和血管内皮生长因子(VEGF)在侵袭性垂体腺瘤组织中表达水平变化相关性研究以及意义。方法:选择承德市中心医院神经外科手术切除的56例垂体腺瘤患者,根据肿瘤侵袭性分为无功能性侵袭性垂体腺瘤(观察组,共38例)和无功能性非侵袭性垂体腺瘤患者(对照组,共18例)。采用实时定量PCR及免疫组织化学法测定观察组及对照组中TNF-α、IL-6和VEGF的表达水平,了解三者在垂体腺瘤侵袭性生长过程中所起的作用。结果:观察组TNF-α、IL-6和VEGF mRNA表达水平均高于对照组(均P<0.05),免疫组织化学染色的结果观察组TNF-α、IL-6和VEGF的表达均高于对照组,差异具有统计学意义(均P<0.05)。结论:垂体腺瘤组织中TNF-α、IL-6和VEGF的高表达可使垂体腺瘤易于侵袭性生长,低表达或无表达可导致侵袭性更低或无侵袭性,TNF-α、IL-6和VEGF可能对垂体腺瘤侵袭性生长起促进作用。Objective:To investigate the correlation between the changes in the expression levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and vascular endothelial growth factor(VEGF)in invasive pituitary adenoma tissues and their significance.Methods:Fifty-six patients with pituitary adenomas surgically resected in the neurosurgery department of Chengde Central Hospital were selected and divided into patients with non-functional invasive pituitary adenomas(observation group,38 patients)and non-functional non-pituitary adenomas(control group,18 patients)according to the invasiveness of the tumor.The expression levels of TNF-α,,IL-6 and VEGF in the observation and control groups were measured by real-time quantitative PCR and immunohistochemistry to understand the role of the three in the aggressive growth of pituitary adenomas.Results:The expression levels of TNF-α,IL-6 and VEGF mRNA were higher in the observation group than in the control group(all P<0.05),and the results of immunohistochemical staining also showed that the expression of TNF-α,IL-6 and VEGF were higher in the observation group than in the control group,and the differences were of high research value and statistical significance(all P<0.05).Conclusion:High expression of TNF-α,IL-6 and VEGF in pituitary adenoma tissues may predispose pituitary adenomas to aggressive growth,and low or no expression may lead to even less aggressive or non-invasive growth.TNF-α,IL-6 and VEGF may contribute to aggressive pituitary adenoma growth.

关 键 词：侵袭性垂体腺瘤 肿瘤坏死因子-Α 白细胞介素-6 血管内皮生长因子 实时聚合酶链反应 免疫组织化学 

分 类 号：R736.4[医药卫生—肿瘤]