子宫内膜癌组织中同源盒蛋白B5表达及对内膜癌细胞生物学影响  

Expression of homeobox protein B5 in endometrial cancer tissue and its influence on the biology of the endometrial cancer cells

作　　者：张洪洋 祝德[1] 赵曼 ZHANG Hongyang;ZHU De;ZHAO Man(Bazhong Central Hospital,Sichuan Province,636000)

机构地区：[1]四川省巴中市中心医院,636000

出　　处：《中国计划生育学杂志》2023年第6期1266-1270,1495,共6页Chinese Journal of Family Planning

摘　　要：目的:探究同源盒蛋白B5(HOXB5)在子宫内膜癌组织中的表达及对子宫内膜癌细胞生物学行为的影响。方法:收集2019年3月-2022年1月本院手术治疗的子宫内膜癌患者内膜癌组织及相对应的癌旁组织标本各35例,qRT-PCR法检测HOXB5表达;将处于对数期的子宫内膜癌细胞Ishikawa随机分为CK组(正常培养)、si-NC组(转染si-NC)、si-HOXB5组(转染si-HOXB5)、pcDNA组(转染pcDNA)、pcDNA-HOXB5组(转染pcDNA-HOXB5),qRT-PCR法检测转染效率;CCK-8法检测细胞增殖;流式细胞仪检测细胞凋亡情况;Transwell实验检测细胞侵袭;划痕实验检测细胞迁移;western blot检测细胞中增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、基质金属蛋白酶-9(MMP-9)蛋白表达;体内移植瘤实验检测肿瘤生长情况。结果:子宫内膜癌组织中HOXB5表达(1.75±0.13)高于癌旁组织(1.00±0.00);与CK组、si-NC组比较,si-HOXB5组Ishikawa细胞中OD_(450)值、侵袭细胞数目、划痕愈合率、PCNA、Bcl-2、MMP-9蛋白表达及裸鼠体内移植瘤质量明显降低,细胞凋亡率、Bax蛋白表达显著升高;与CK组、pcDNA组比较,pcDNA-HOXB5组Ishikawa细胞中OD_(450)值、侵袭细胞数目、划痕愈合率、PCNA、Bcl-2、MMP-9蛋白表达及裸鼠体内移植瘤质量显著升高,细胞凋亡率、Bax蛋白表达明显降低(均P<0.05)。结论:HOXB5在子宫内膜癌组织中高表达,沉默HOXB5可抑制Ishikawa细胞增殖、侵袭和迁移,促进细胞凋亡。Objective:To investigate the expression of homeobox protein B5(HOXB5)in endometrial cancer tissue,and to study its influences on the biological behavior of the endometrial cancer cells.Methods:The 35specimens of the endometrial cancer tissue and 35specimens of the corresponding paracancerous tissue were collected from the patients with endometrial cancer.The expression of HOXB5in the endometrial cancer tissue and in the paracancerous tissue was detected by qRT-PCR.The endometrial cancer cells Ishikawa in log phase were randomly divided into CK group(cells were normal cultured),si-NC group(cells were transfected with si-NC),si-HOXB5group(cells were transfected with si-HOXB5),pcDNA group(cells were transfected with pcDNA),and pcDNA-HOXB5group(cells were transfected with pcDNA-HOXB5).The transfection efficiency of these groups was detected by qRT-PCR.The cell proliferation was detected by CCK-8assay,and the cell apoptosis was detected by flow cytometry.Transwell assay was applied to detect the cell invasion,and Scratch assay was applied to detect the cell migration.Western blot was applied to detect the expressions of proliferating cell nuclear antigen(PCNA),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and matrix metalloproteinase-9(MMP-9)protein in the cells.In vivo xenograft experiment was used to detect the situation of tumor growth.Results:The expression of HOXB5(1.75±0.13)in the endometrial cancer tissues was significantly higher than that(1.00±0.00)in the paracancerous tissues(P<0.05).The OD_(450)value,the number of invasive cells,the wound healing rate,the protein expressions of PCNA,Bcl-2,and MMP-9in the Ishikawa cells,and the quality of the transplanted tumor of the nude mice in si-HOXB5group were significantly lower than those in CK group and in si-NC group.The apoptosis rate and the protein expression of Bax in si-HOXB5group were significantly higher than those in CK group and in si-NC group.The OD_(450)value,the number of invasive cells,the wound healing rate,the protein expression rateS of

关 键 词：子宫内膜癌组织 同源盒蛋白B5 细胞生物学 沉默 凋亡 迁移 增值 

分 类 号：R737.33[医药卫生—肿瘤]