基于生物信息学分析MTMR2基因在HCC中的表达及临床意义  

Analysis of the expression and clinical significance of MTMR2 in HCC based on bioinformatics

作　　者：李东旭 柳家翠 段怡平 陈梁玥 马甜甜 朱翠雯 张晓洋 喻明霞[2] 唐艳 LI Dongxu;LIU Jiacui;DUAN Yiping;CHEN Liangyue;MA Tiantian;ZHU Cuiwen;ZHANGXiaoyang;YU Mingxia;TANG Yan(Department of General Practice,Tianyou Hospital Affiliated to Wuhan University of Science and Technology,Wuhan,Hubei,China,430064;Department of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan,Hubei,China,430071)

机构地区：[1]武汉科技大学附属天佑医院全科医学科,湖北武汉430064 [2]武汉大学中南医院检验科,湖北武汉430071

出　　处：《分子诊断与治疗杂志》2023年第5期858-862,共5页Journal of Molecular Diagnostics and Therapy

基　　金：国家自然科学基金(81472033);湖北省医学青年后备人才(青年拔尖人才)(HB20200409);湖北省卫生和计划生育委员会联合基金项目(WJ2018H0028);湖北省卫生健康科研基金资助(WJ2019M203);武汉市应用基础研究(2017060201010171);武汉大学医学部教学研究项目(2020048);武汉大学中南医院转化医学及交叉学科研究联合基金资助项目(ZNJC202010);武汉大学中南医院科技创新培育基金(cxpy2019051、cxpy2018031);武汉大学大学生创新项目(202110486085、W2021303003、W2020303005、W2020301001、MS2017045、S2018301747)。

摘　　要：目的研究肌管蛋白相关蛋白2基因(MTMR2)在肝细胞癌(HCC)中的表达及与患者临床病理特征、免疫浸润以及疾病预后的关系。方法从TCGA数据库中下载HCC的基因表达数据及患者的临床病理信息,分析MTMR2在HCC组织和正常组织之间的表达差异。使用χ^(2)检验分析MTMR2的表达与临床病理信息的联系。利用Kaplan-Meier法和单因素、多因素Cox风险回归模型分析MTMR2在HCC中的预后相关性及预后价值。利用基因富集分析探索MTMR2在HCC中可能参与的分子信号途径。使用R 4.1.1软件评估MTMR2表达与免疫浸润的相关性。结果MTMR2在HCC样本中的表达显著上调(P<0.05),Kaplan-Meier分析显示MTMR2高表达组患者的总体生存率显著低于低表达组,差异有统计学意义(P<0.05)。MTMR2表达与患者性别、T分期、grade分级以及stage分期相关(P<0.05)。富集分析结果显示,MTMR2主要参与卵母细胞减数分裂、WNT信号通路和磷酸肌醇代谢通路等通路。MTMR2表达主要与癌组织内中性粒细胞的浸润相关(P<0.05)。结论MTMR2在HCC中表达显著上调,与HCC患者的不良预后有关,可作为HCC的独立预后因子。Objective To explore the relationship between the expression of myotubularin⁃related protein 2(MTMR2)in hepatocellular carcinoma(HCC)and clinicopathological features,immune infiltration,and prognosis.Methods The HCC gene expression data and clinicopathological information of patients were downloaded from TCGA database.The expression difference of MTMR2 between HCC tissues and normal tissues was analyzed.The relationship between MTMR2 expression and clinicopathological features was analyzed by chi⁃square test.Kaplan Meier method and univariate and multivariate Cox risk regression models were used to analyze the prognostic correlation and prognostic value of MTMR2 in HCC.Gene enrichment analysis was used to explore the molecular pathway of MTMR2 in hepatocellular carcinoma.The association between MTMR2 expression and the abundance of infiltrating immune was evaluated by R 4.1.1 software.Results The expression level of MTMR2 in HCC samples was significantly up⁃regulated(P<0.05).Kaplan Meier analysis showed that the overall survival rate of patients in the high expression group was significantly lower than the low expression group(P<0.05).The expression level of MTMR2 was correlated with gender,tumor grade,T stage and clinical stage(P<0.05).The results of GSEA enrichment analysis suggest that MTMR2 is mainly involved in oocyte meiosis,WNT signal pathway and inositol phosphate metabolism pathway.The expression of MTMR2 was mainly related to the infiltration of neutrophils in HCC tissues(P<0.05).Conclusion The expression of MTMR2 is significantly up regulated in HCC,which is related to the poor prognosis of HCC patients and can be used as an independent prognostic factor for HCC.

关 键 词：肝细胞癌 MTMR2 TCGA 免疫浸润 

分 类 号：R735.7[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]