液相色谱-串联质谱法测定毛发中甲基苯丙胺含量的实验设计  

Experimental Design of the Determination of Methamphetamine in Hair by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：刘太硕 胡骏杰 陈云龙 余晓冬 孔璇凤[3] 戴洁[3] Liu Taishuo;Hu Junjie;Chen Yunlong;Yu Xiaodong;Kong Xuanfeng;Dai Jie(College of Forensic Medicine and Laboratory Medicine,Jining Medical University,Jining 272067;State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023;National Demonstration Center for Experimental Chemistry Education,Nanjing University,Nanjing 210023,China)

机构地区：[1]济宁医学院法医学与医学检验学院,山东济宁272067 [2]南京大学化学化工学院生命分析化学国家重点实验室,江苏南京210023 [3]南京大学国家级化学实验教学示范中心,江苏南京210023

出　　处：《广东化工》2023年第11期186-188,200,共4页Guangdong Chemical Industry

基　　金：2021年基础学科拔尖学生培养计划2.0课题(20212044);江苏省高等教育教改研究课题(2021JSJG027);南京大学本科实验教学质量提升计划;济宁医学院大学生创新训练计划项目(cx2021050)。

摘　　要：设计了仪器分析综合实验——液相色谱-串联质谱法(LC-MS/MS)测定毛发中甲基苯丙胺含量。实验内容包括不同前处理方法的操作与对比,毛发中甲基苯丙胺的定性分析、定量测定以及图谱中代谢物等相关信息的发掘与解读。该实验将毒物鉴定技术与LC-MS/MS法结合,学以致用,与前沿和应用接轨。本实验的设立,将加深学生对LC-MS/MS的了解,促进学生构建完整实验体系,增强科研意识与创新能力。A comprehensive instrumental analysis experiment,the determination of methamphetamine in hair by liquid chromatography-tandem mass spectrometry(LC-MS/MS),is designed.The experimental contents include the operation and comparison of different methods for the extraction of methamphetamine in hair samples,qualitative and quantitative results of target analyte,and the discovery and interpretation of the metabolite’s information from the extracted ion chromatograms.This experiment combines the technology of toxicological analysis and the method of LC-MS/MS,and applies what we have learned to the forefront.It can deepen the students’understanding of LC-MS/MS,promote to build a complete experimental system,and enhance the scientific research awareness and innovation ability.

关 键 词：仪器分析 综合实验 液相色谱-串联质谱 毛发 甲基苯丙胺 

分 类 号：TQ[化学工程]