细胞外信号调节蛋白激酶在颞下颌关节骨关节炎髁突软骨退变中的作用  

Effect of extracellular signal-regulated kinase on condylar cartilage degeneration of temporomandibular joint osteoarthritis

作　　者：任昊喆 牟婧 赵子璇 康馨予 张勉 苗辉 于世宾 何峰 REN Haozhe;MOU Jing;ZHAO Zixuan;KANG Xinyu;ZHANG Mian;MIAO Hui;YU Shibin;HE Feng(Stomatological College,Xi'an Jiaotong University,Xi'an 710004,China;Lintong Medical Rehabilitation Center,Xi'an 710600,China;State Key Laboratory of Military Stomatology,National Clinical Research Center for Oral Diseases,Shaanxi International Joint Research Center for Oral Diseases,Department of Oral Anatomy and Physiology,Stomatological Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]西安交通大学口腔医学院,陕西西安710004 [2]临潼康复疗养中心,陕西西安710600 [3]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学口腔医院口腔解剖生理学教研室,陕西西安710032

出　　处：《空军军医大学学报》2023年第6期490-495,共6页Journal of Air Force Medical University

基　　金：国家自然科学基金面上项目(81970953);陕西省重点研发计划一般项目(2021SF-046)。

摘　　要：目的探究细胞外信号调节蛋白激酶(ERK)在小鼠颞下颌关节骨关节炎(TMJOA)髁突软骨退变中的作用。方法C57BL/6J雌性小鼠36只,随机分成假手术对照组(Sham组)和单侧前牙反[牙合]组(UAC组),每组于造模3、7、11周后各取6只处死,取颞下颌关节(TMJ)。另18只小鼠随机分成假手术对照+关节腔注射PBS组(Sham+PBS组)、单侧前牙反[牙合]+关节腔注射PBS组(UAC+PBS组)和单侧前牙反[牙合]+关节腔注射ERK抑制剂PD98059组(UAC+PD98059组),11周后处死,取TMJ。HE和番红O染色评价软骨退变程度;RT-PCR检测软骨基质(Aggrecan)、增殖相关分子(Pcna)和分化/降解分子(Mmp13)的mRNA表达水平;免疫组化染色检测髁突软骨中磷酸化ERK(p-ERK)表达。结果与同期Sham组相比,UAC组软骨厚度、番红O阳性面积比、Aggrecan和Pcna mRNA表达显著降低(P<0.01),Mmp13 mRNA表达、p-ERK阳性细胞率显著升高(P<0.01)。与UAC+PBS组相比,UAC+PD98059组软骨厚度、番红O阳性面积比、Aggrecan和Pcna mRNA表达显著升高(P<0.01),Mmp13 mRNA表达显著降低(P<0.01)。结论ERK参与了TMJOA的软骨退行性变进程,阻断ERK相关通路可以显著逆转髁突软骨退行性变进程。Objective To explore the effect of extracellular signal-regulated kinase(ERK)on condylar cartilage degeneration of temporomandibular joint osteoarthritis(TMJOA)in mice.Methods Thirty-six female C57BL/6J mice were randomly divided into sham-operated group(Sham group)and unilateral anterior crossbite group(UAC group).Six mice from each group were sacrificed at 3,7 and 11 weeks after modeling and temporomandibular joint(TMJ)was extracted.Another 18 mice were randomly divided into Sham+PBS group,UAC+PBS group,and UAC+PD98059 group,and were sacrificed 11 weeks later.HE staining and safranin O staining were used to evaluate the degree of condylar cartilage degeneration.RT-PCR was used to detect the mRNA expression levels of Aggrecan,Pcna and Mmp 13.Immunohistochemical staining was used to detect the expression of phosphorylated ERK(p-ERK)in condylar cartilage.Results Compared with Sham group at the same time point,there was a significant decrease in the thickness of cartilage,the percentages of safranin O-positive area and the mRNA expression of Aggrecan and Pcna(P<0.01),and a significant increase in the percentages of p-ERK-positive chondrocytes and the mRNA expression of Mmp 13 in UAC group(P<0.01).Compared with UAC+PBS group,there was a significant increase in the thickness of cartilage,the percentages of safranin O-positive area and the mRNA expression of Aggrecan and Pcna(P<0.01),and a significant decrease in the mRNA expression of Mmp 13 in UAC+PD98059 group(P<0.01).Conclusion ERK is involved in the condylar cartilage degeneration of TMJOA,which can be significantly reversed by blocking ERK-related pathways.

关 键 词：细胞外调节蛋白激酶 髁突 软骨 骨关节炎 退行性变 

分 类 号：R782.6[医药卫生—口腔医学]