Improving the Efficiency and Orthogonality of Genetic Code Expansion  

作　　者：Xian Fu Yijian Huang Yue Shen 

机构地区：[1]BGI-Shenzhen,Shenzhen 518083,China [2]Guangdong Provincial Key Laboratory of Genome Read and Write,Shenzhen 518120,China [3]School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,China [4]Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

出　　处：《BioDesign Research》2022年第1期248-260,共13页生物设计研究（英文）

基　　金：the National Key Research and Development Program of China(No.2018YFA0900100);National Natural Science Foundation of China(No.31901029 to X.F.and No.31800078 to Y.S.);Natural Science Foundation of Guangdong Province,China(No.2021A1515010995 to X.F.);Guangdong Provincial Key Laboratory of Genome Read and Write(No.2017B030301011).

摘　　要：The site-specific incorporation of the noncanonical amino acid(ncAA)into proteins via genetic code expansion(GCE)has enabled the development of new and powerful ways to learn,regulate,and evolve biological functions in vivo.However,cellular biosynthesis of ncAA-containing proteins with high efficiency and fidelity is a formidable challenge.In this review,we summarize up-to-date progress towards improving the efficiency and orthogonality of GCE and enhancing intracellular compatibility of introduced translation machinery in the living cells by creation and optimization of orthogonal translation components,constructing genomically recoded organism(GRO),utilization of unnatural base pairs(UBP)and quadruplet codons(four-base codons),and spatial separation of orthogonal translation.

关 键 词：TRANSLATION HAS BASE 

分 类 号：Q3[生物学—遗传学]