Polycaprolactone strengthening keratin/ bioactive glass composite scaffolds with double cross-linking networks for potential application in bone repair  

作　　者：Liying Sun Shan Li Kaifeng Yang Junchao Wang Zhengjun Li Nianhua Dan 

机构地区：[1]National Engineering Research Center of Clean Technology in Leather Indus-try,Sichuan University,Chengdu,China [2]Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu,China

出　　处：《Journal of Leather Science and Engineering》2022年第1期1-13,共13页皮革科学与工程（英文）

基　　金：National Natural Science Foundation of China(No.21376153);Fundamental Research Funds for the Central University.

摘　　要：In this study, we aimed at constructing polycaprolactone (PCL) reinforced keratin/bioactive glass composite scaffolds with a double cross-linking network structure for potential bone repair application. Thus, the PCL-keratin-BG com-posite scaffold was prepared by using keratin extracted from wool as main organic component and bioactive glass (BG) as main inorganic component, through both cross-linking systems, such as the thiol-ene click reaction between abundant sulfhydryl groups of keratin and the unsaturated double bond of 3-methacryloxy propyltrimethoxy silane (MPTS), and the amino-epoxy reaction between amino groups of keratin and the epoxy group in (3-glycidoxymethyl) methyldiethoxysilane (GPTMS) molecule, along with introduction of PCL as a reinforcing agent. The success of the thiol-ene reaction was verified by the FTIR and 1H-NMR analyses. And the structure of keratin-BG and PCL-keratin-BG composite scaffolds were studied and compared by the FTIR and XRD characterization, which indicated the successful preparation of the PCL-keratin-BG composite scaffold. In addition, the SEM observation, and contact angle and water absorption rate measurements demonstrated that the PCL-keratin-BG composite scaffold has interconnected porous structure, appropriate pore size and good hydrophilicity, which is helpful to cell adhesion, differentiation and prolifera-tion. Importantly, compression experiments showed that, when compared with the keratin-BG composite scaffold, the PCL-keratin-BG composite scaffold increased greatly from 0.91 ± 0.06 MPa and 7.25 ± 1.7 MPa to 1.58 ± 0.21 MPa and 14.14 ± 1.95 MPa, respectively, which suggesting the strong reinforcement of polycaprolactone. In addition, the biomineralization experiment and MTT assay indicated that the PCL-keratin-BG scaffold has good mineralization abil-ity and no-cytotoxicity, which can promote cell adhesion, proliferation and growth. Therefore, the results suggested that the PCL-keratin-BG composite scaffold has the potential as a candidate for application in

关 键 词：KERATIN Bioactive glass POLYCAPROLACTONE Double cross-linking networks Bone regeneration 

分 类 号：R318.08[医药卫生—生物医学工程] TB332[医药卫生—基础医学]