基于MSER仿射变换配准的农用柔性pH芯片性能优化  

Effect of MSER Affine Transformation Registration on Agro-pH Flexible Chip

作　　者：张淼[1,2] 王丽茹 李浩榛 路逍 刘刚[1,2] ZHANG Miao;WANG Liru;LI Haozhen;LU Xiao;LIU Gang(Key Laboratory of Smart Agriculture Systems,Ministry of Education,China Agricultural University,Beijing 100083,China;Key Laboratory of Agricultural Information Acquisition Technology,Ministry of Agriculture and Rural Affairs,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学智慧农业系统集成研究教育部重点实验室,北京100083 [2]中国农业大学农业农村部农业信息获取技术重点实验室,北京100083

出　　处：《农业机械学报》2023年第5期288-294,386,共8页Transactions of the Chinese Society for Agricultural Machinery

基　　金：浙江省重点研发计划项目(2020C02017);云南省院士工作站项目(2015IC16);中央高校基本科研业务费专项资金项目(2022TC161)。

摘　　要：针对柔性pH芯片复合结构多层打印制备过程的配准偏差严重,限制其农用pH值在线监测精度及稳定性问题,比较分析了MSER仿射变换模型对“叠层复合结构”柔性pH芯片性能的影响,分析了配准后柔性pH芯片在基质培番茄根系pH值在线监测应用中的可行性。试验结果表明:MSER仿射变换模型配准后,农用柔性芯片加工最小线宽和线间距分别为90、500μm,芯片加工平均相对误差可控制在25%以内;配准后,农用柔性pH传感器灵敏度达到了每单位pH值-61.9 mV,响应范围为2.0~10.0,与商用玻璃pH电极的测量绝对误差小于0.15,相对误差小于4.1%;基质培番茄根系pH值监测试验中,自制柔性芯片与商用pH电极的测量结果具有较好一致性,同步测定结果的绝对误差小于0.09,相对误差小于1.5%,RMSE仅为0.05。MSER仿射变换配准方法可有效提高“叠层复合结构”农用打印柔性传感芯片的制备精度及传感检测准确性。In order to meet the demand of sustainable precision agriculture for efficient and real-time acquisition of agricultural production information,flexible pH sensors have been widely studied for their small structure,easy integration and strong mechanical performance.However,the problem of deviation between multi-layer printing in the preparation process has not been solved.Therefore,aiming at improving the accuracy of sensor preparation,the registration performance of affine transformation model was discussed based on MSER.The model verification and precision test experiments were carried out to verify the feasibility of the registration model and the accuracy that the registration can achieve.The model was applied to the preparation of the flexible pH sensor chip,and finally the feasibility of the registered flexible pH sensor chip in the online monitoring of tomato organic matrix pH was verified.The experimental results showed that the accuracy of the model can reach the minimum line width of 90μm.Above 90μm,the RMSE and MAE were not more than 140μm,MRE was 3.33%~22.22%.The minimum line spacing was 500μm.Above 500μm,the RMSE and MAE were not more than 160μm,MRE was 11.67%~24%.The sensitivity of the flexible pH sensor prepared by this registration method was-61.9 mV per unit pH value,and the response range was 2.0~10.0.Compared with the standard glass pH sensor,the absolute error was less than 0.15 and the relative error was less than 4.1%.The slope of the fitting curve was 0.97 and the determination coefficient R2 was 0.99.The fitting degree and accuracy was high.In the pH monitoring test of tomato organic matrix,the measurement results of the flexible and standard pH sensors had good consistency.The absolute error of the synchronous measurement results was less than 0.09,the relative error was less than 1.5%,and the RMSE between the pH monitoring results for a continuous week was only 0.05.The registered flexible pH sensor chip showed a good agricultural application prospect because of its miniaturization

关 键 词：pH值在线监测 柔性pH芯片 最大稳定极值区域 仿射变换 3D打印多层配准 

分 类 号：S126[农业科学—农业基础科学]