Steroid hormone 20-hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae  

作　　者：Sheng-Yu Zhang Han Gao Ankarjan Askar Xing-Peng Li Guo-Cai Zhang Tian-Zhong Jing Hang Zou Hao Guan Yun-He Zhao Chuan-Shan Zou 

机构地区：[1]School of Forestry,Northeast Forestry University,Harbin,China [2]Beiua University,Jilin,Chia

出　　处：《Insect Science》2023年第3期771-788,共18页昆虫科学（英文版）

基　　金：the Natural Science Foundation of Heilongjiang Province(LH2021C010);National Undergraduate Training Programs for Innovations(202110225005);Fundamental Research Fund for the Central Universities(2572022DS09,2572020DR09,2572020DR02).

摘　　要：The steroid hormone 20-hydroxyecdysone(20E)has been described to regulate fat body lipid metabolism in insects,but its accurate regulatory mechanism,especially the crosstalk between 20E-induced lipid metabolism and gluconeogenesis remains largely unclear.Here,we specially investigated the effect of 20E on lipid metabolism and gluconeogenesis in the fat body of Hyphantria cunea larvae,a notorious pest in forestry.Lipidomics analysis showed that a total of 1907 lipid species were identified in the fat body of H.cunea larvae assigned to 6 groups and 48 lipid classes.The differentially abundant lipids analysis showed a significant difference between 20E-treated and control samples,indicating that 20E caused a remarkable alteration of lipidomics profiles in the fat body of H.cunea larvae.Further studies demonstrated that 20E accelerated fatty acidβ-oxidation,inhibited lipid synthesis,and promoted lipolysis.Meanwhile,the activities of pyruvate carboxylase,phosphoenolpyruvate carboxykinase,fructose-1,6-bisphosphatase,and glucose-6-phosphatase were dramatically suppressed by 20E in the fat body of H.cunea larvae.As well,the transcriptions of genes encoding these 4 rate-limiting gluconeogenic enzymes were significantly downregulated in the fat body of H.cunea larvae after treatment with 20E.Taken together,our results revealed that 20E disturbed fat body lipid homeostasis,accelerated fatty acidβ-oxidation and promoted lipolysis,but negatively regulated gluconeogenesis in H.cunea larvae.The findings might provide a new insight into hormonal regulation of glucose and lipid metabolism in insect fat body.

关 键 词：20-HYDROXYECDYSONE Hyphantria cunea fat body lipidomics analysis lipid metabolism GLUCONEOGENESIS 

分 类 号：O62[理学—有机化学]