Sanwu Baisan decoction(三物白散)inhibits colorectal cancer progression in mice by remodeling gut microbiota and tumorigenesis  

作　　者：JIANG Yiqian ZHOU Xibin PU Wenyuan ZHOU Chunxiang 

机构地区：[1]Department of Febrile Disease,Basic Medicine College,Nanjing University of Chinese Medicine,Nanjing 210046,China [2]Department of Traditional Chinese Medicine,Second Military Medical University,Shanghai 200433,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第3期466-473,共8页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation-funded Project:the Biological Mechanism of Lingguizhugan Decoction Treating Alzheimer’s Disease via Regulating Brain Lymphatic Drainage System(No.82074504);the Priority Academic Program Development of Jiangsu Higher Education Institutions(Integration of Chinese and Western Medicine);Natural Science Foundation of the Jiangsu Higher Education Institutions-funded Project:to Explore the Mechanism of Sanwubai San Regulating Gut Microbiota Against Colorectal Cancer By IL-23/ΓδT17/IL-17 Axis(19KJB360003)。

摘　　要：OBJECTIVE:To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction(三物白散,SWB)in treatment of colorectal cancer(CRC)in mice.METHODS:Therapeutic effect was evaluated based on body weight gain,tumor volume,tumor growth inhibition rate,and histological changes and apoptosis in the tumor tissues.Anti-tumor immunity was studied by measuring plasma levels of anti-tumor cytokines,interleukin 6(IL-6),interleukin 17(IL-17),and interferonγ(IFN-γ).Gut morphological changes were evaluated by histological staining and tight junction proteins expressions.Gut microbiota composition was analyzed by 16S r RNA gene sequencing.Classical toll-like receptor 4(TLR-4)/cyclooxygenase 2(COX-2)/prostaglandin E2(PGE-2)pathway was examined in colon tissue and tumor samples.RESULTS:SWB presented high anti-tumor efficacy of CRC in mice,which manifested as decreased tumor volume and increased tumor growth inhibition rate.This anti-tumor effect of SWB was associated with elevated plasma levels of anti-tumor immune cytokines(IL-6,IL-17,and IFN-γ).Further studies showed that SWB also increases the expression of occluding and promotes the abundance of gut probiotics,Clostridium_XIVa,Enterorhabdus,and uncultured_bacterium.Moreover,results suggested that the anti-tumor effects of SWB might associate with inducing cancer cell apoptosis and inhibiting the TLR-4/COX-2/PGE-2 pathway in both colon tissue and tumor samples.CONCLUSION:SWB shows strong anti-tumor efficiency in mice with colorectal carcinoma,possibly through promoting the secretion of anti-tumor immune cytokines,inducing cancer apoptosis,maintaining the gut microbiota,and inhibiting tumorigenesis by inhibiting the TLR-4/COX-2/PGE-2 pathway.

关 键 词：colorectal neoplasms CARCINOGENESIS apoptosis gastrointestinal microbiome DYSBIOSIS Sanwu Baisan decoction 

分 类 号：R285.5[医药卫生—中药学]