安肠汤对重症溃疡性结肠炎大鼠肠道菌群及钙卫蛋白的影响  

Effect of Anchang Decoction on intestinal flora and fecal calcitonin in rats with severe ulcerative colitis

作　　者：于璇 赖斯华 高丹 朱智仁 唐泽彬 司林光 农评皓 孙平良[2] Xuan Yu;Sihua Lai;Dan Gao;Zhiren Zhu;Zebin Tang;Linguang Si;Pinghao Nong;Pingliang Sun(Graduate School of Guangxi University of Chinese Medicine,Nanning 530200,China;Department of General Surgery,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]广西中医药大学研究生院,南宁530200 [2]广西中医药大学第一附属医院普通外科,南宁530023

出　　处：《国际中医中药杂志》2023年第6期714-718,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81660795);广西自然科学基金项目(2017JJA140532z,2018JJA140199);广西医疗卫生适宜技术开发与推广应用项目(S2017044);广西中医药大学引进博士科研启动基金项目(2019BS032)。

摘　　要：目的基于益生菌探究安肠汤对重症溃疡性结肠炎模型大鼠肠道菌群及粪钙卫蛋白的影响。方法取50只大鼠制备重症溃疡性结肠炎模型。将造模成功大鼠按随机数字表法分为模型组、安肠汤低剂量组、安肠汤中剂量组、安肠汤高剂量组、丽珠肠乐组、美沙拉嗪组,每组5只。另设空白组5只。丽珠肠乐组大鼠灌胃丽珠肠乐混悬液(0.5亿活菌/ml),安肠汤低、中、高剂量组分别灌胃安肠汤1、5、10 ml/(kg·d),美沙拉嗪组灌胃美沙拉嗪混悬液(10.5 mg/100 g)。空白组和模型组大鼠灌胃等体积生理盐水,连续灌胃7 d。测定大鼠给药前后体重及疾病活动指数(DAI)评分,采用PCR法检测大鼠粪便中乳杆菌属及双歧杆菌属菌群16s rDNA,采用ELISA法检测大鼠粪便钙卫蛋白水平。结果与模型组比较,各给药组大鼠体重升高(P<0.05),DAI评分降低(P<0.05),粪便中钙卫蛋白水平降低(P<0.05),各给药组肠道乳杆菌属、双歧杆菌属水平比较差异无统计学意义(P>0.05)。结论安肠汤可通过调控重症溃疡性结肠炎大鼠钙卫蛋白水平,改善肠道微生态,发挥治疗作用。Objective To investigate the effects of Anchang Decoction on intestinal microflora and faecal calprotectin(FC)in rats with severe ulcerative colitis based on probiotics.Methods Totally 50 rats were taken to prepare a model of severe ulcerative colitis.The rats who successfully modeled were divided into model group,Anchang Decoction low-,medium-,high-dosage groups,Lizhu Changle group,and mesalazine group,with 5 rats in each group.Another 6 rats were set and the blank group.Lizhu Changle group received Lizhu Changle suspension(containing 50 million live bacteria/ml)for gavage;Anchang Decoction low-,medium-,high-dosage groups received Anchang Decoction 1,5,10 ml/(kg·d)for gavage;Mesalazine group received Mesalazine suspension(10.5 mg/100 g)for gavage.Rats in the blank group and model group were gavaged with the same volume of normal saline for 7 days.Body mass and disease activity index(DAI)before and after administration were measured.16s rDNA of lactobacillus and bifidobacterium in feces of rats was detected.FC content of rats was detected by ELISA.Results Compared with the model group,the weight of rats in each administration group increased(P<0.05),DAI score decreased(P<0.05),and the level of calprotectin in feces decreased(P<0.05),and there was no significant difference in the levels of lactobacillus and bifidobacterium in each administration group(P>0.05).Conclusion Anchang Decoction can improve the intestinal micro-ecology by regulating the level of calprotectin in rats with severe ulcerative colitis,thereby playing a therapeutic role.

关 键 词：结肠炎 溃疡性 乳杆菌属 双歧杆菌属 粪钙卫蛋白 益生菌 安肠汤 大鼠 

分 类 号：R285.5[医药卫生—中药学]