SAHA对裸鼠人乳腺癌细胞MCF-7移植瘤的影响  

Effect of SAHA on human breast cancer cell MCF-7 transplanted tumor in nude mice

作　　者：于日成 焦凯仑 何涛[2] YU Yicheng;JIAO Kailun;HE Tao(Graduate School of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,Inner Mongolia,China;Department of Breast Surgery,the First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology)

机构地区：[1]内蒙古科技大学包头医学院研究生院,内蒙古包头014040 [2]内蒙古科技大学包头医学院第一附属医院乳腺外科

出　　处：《包头医学院学报》2023年第6期40-46,共7页Journal of Baotou Medical College

摘　　要：目的:研究辛二酰苯胺异羟肟酸(SAHA)是否可以调节RECK蛋白使MMP-2、MMP-9在动物体内表达发生改变,从而达到抑制乳腺癌细胞生长、增殖和转移,改善预后的作用,提供乳腺癌临床治疗新思路。方法:乳腺癌细胞MCF-7培养后,被移植到裸鼠皮下,制成动物模型。将裸鼠成瘤模型分为对照组(生理盐水0.4 mL/kg)和实验组(SAHA 0.42 mg/kg),成瘤模型建立后,ELISA方法检测裸鼠体内IL-6的含量。断颈法处死裸鼠,取肿瘤组织称重并计算脏器指数。取肿瘤组织做HE病理性染色。IHC、WB检测MMP-2、MMP-9、RECK在肿瘤组织中的表达。结果:与对照组相比,实验组IL-6含量、肿瘤重量及脏器指数降低(P<0.01)。肿瘤组织HE染色显示,实验组以中重度坏死为主,对照组中以轻中度坏死为主。IHC检测显示,与对照组相比,实验组RECK阳性表达较高,MMP-2、MMP-9阳性表达较低(P<0.05)。WB检测显示,与对照组相比,实验组RECK表达较高,MMP-2、MMP-9表达较低(P<0.01)。结论:SAHA可在动物体内通过提高RECK蛋白的表达来降低MMP-2、MMP-9的表达,从而抑制了人乳腺癌MCF-7细胞的侵袭和转移。Objective:To study whether suberoylanilide hydroxamic acid(SAHA)can regulate RECK protein to change the expression of MMP-2 and MMP-9 in animals,so as to inhibit the growth,proliferation and metastasis of breast cancer cells,improve the prognosis,and provide new ideas for clinical treatment of breast cancer.Methods:The breast cancer cell MCF-7 was cultured and transplanted subcutaneously into nude mice to make an animal model.The nude mouse tumor model was divided into control group(normal saline 0.4 mL/kg)and experimental group(SAHA 0.42 mg/kg).After the tumor model was established,the content of IL-6 in nude mice was detected by ELISA.The nude mice were sacrificed by neck cutting method,and the tumor tissues were weighed and the organ index was calculated.HE pathological staining was performed on tumor tissues.The expression of MMP-2,MMP-9 and RECK in tumor tissues was detected by IHC and WB.Results:Compared with the control group,the IL-6 content,tumor weight and organ index of the experimental group decreased(P<0.01).HE staining of tumor tissue showed that the experimental group was mainly moderate to severe necrosis,while the control group was mainly mild to moderate necrosis.IHC test showed that compared with the control group,the positive expression of RECK in the experimental group was higher,and the positive expression of MMP-2 and MMP-9 was lower(P<0.05).WB test showed that compared with the control group,the expression of RECK in the experimental group was higher,and the expression of MMP-2 and MMP-9 was lower(P<0.01).Conclusion:SAHA can reduce the expression of MMP-2 and MMP-9 by increasing the expression of RECK protein in animals,thus inhibiting the invasion and metastasis of human breast cancer MCF-7 cell.

关 键 词：MCF-7细胞 乳腺癌 辛二酰苯胺异羟肟酸 MMP-2 MMP-9 RECK 

分 类 号：R737.9[医药卫生—肿瘤]