响应面设计优化铁皮石斛组织培养体系  

Optimization of the Tissue Culture System of Dendrobium Officinale by Response Surface Methodology

作　　者：杨艳君[1] 陈林晶[1] 邵青玲[1] 马晓丽[1] YANG Yan-jun;CHEN Lin-jing;SHAO Qing-ling;MA Xiao-li(Department of Biological Sciences and Technology,Jinzhong University,Jinzhong Shanxi030619,China)

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030619

出　　处：《晋中学院学报》2023年第3期62-67,共6页Journal of Jinzhong University

基　　金：山西省自然科学基金(面上项目)“基于根际微生物探讨谷子高产优质的作用机制及菌肥推广”(202203021221226);晋中学院“1331工程”技术创新团队——旱作农业谷子绿色生产技术创新团队(jzxyjscxtd202108)。

摘　　要：以铁皮石斛叶片为试验材料,采用三因素五水平响应面设计对铁皮石斛愈伤组织诱导、分化和继代增殖三个阶段激素6-BA(6-苄氨基嘌呤)、NAA(萘乙酸)和2,4-D(2,4-二氯苯氧乙酸)的配方进行优化.生根培养以1/2 MS培养基对比了两种激素IBA(0.5 mg/L、1.0 m/L、1.5 mg/L和2.0 mg/L)、NAA (0.3 mg/L、0.4 mg/L、0.5 mg/L和0.6 mg/L)及NAA+活性炭(2g/L)对其生根率的影响,从而优化各阶段的配方,建立起一个比较完善的铁皮石斛培养体系.通过回归分析得到:愈伤组织的最优激素浓度配比为0.16 mg/L 6-BA+0.40 mg/L NAA+1.45 mg/L2,4-D;不定芽分化的最优激素浓度配比为0.19 mg/L 6-BA+0.15 mg/L NAA+0.54 mg/L2,4-D;增殖的最优激素配比为2.17 mg/L 6-BA+0.03 mg/L NAA+0.19 mg/L 2,4-D.生根培养的较好培养基配方是1/2 MS+0.5 mg/L NAA+2 g/L活性炭.In this experiment,using blades of Dendrobium officinale as the test material,a three-factor and five-level response surface design was used to optimize the formulation of hormones 6-BA(6-benzylaminoprine),NAA(naphthyl acetic acid)and 2,4-D(2,4-dichlorophenoxyacetic acid)in the three stages of callus induction,differentiation and subproliferation of Dendrobium officinale.In rooting culture the effects of the two hormones IBA(0.5 mg/L,1.0 mg/L,1.5 mg/L,2.0 mg/L),NAA(0.3 mg/L,0.4 mg/L,0.5 mg/L,0.6 mg/L)and NAA+activated carbon(2 g/L)on Dendrobium officinale rooting rate were compared,so as to optimize the best formula at each stage and establish a relatively complete cultivation system of Dendrobium officinale.Multivariate quadratic regression analysis indicated that the optimal hormone concentration ratio of callus is 0.16 mg/L 6-BA+0.4 mg/L NAA+1.45 mg/L.The optimal hormone for adventitious bud differentiation concentration ratio is 0.19 mg/L 6-BA+0.15 mg/L NAA+0.54 mg/L 2,4-D.The optimal hormone ratio for proliferation is 2.17 mg/L 6-BA+0.03 mg/L NAA+0.19 mg/L 2,4-D.A better medium formulation for rooting culture is 1/2 MS+0.5 mg/L NAA+2 g/L activated carbon.Thus,the tissue culture system of Dendrobium officinale was established and the medium formula of tissue culture was optimized.The reference was provided for the rapid propagation,development and utilization of Dendrobium officinale.

关 键 词：铁皮石斛 组织培养 响应面设计 优化 植物激素 

分 类 号：Q94-3[生物学—植物学]