High-throughput sequencing to evaluate the effects of methamphetamine on the succession of the bacterial community to estimate the postmortem interval  

作　　者：Shujuan Wang Wei Chen Yanjie Shang Lipin Ren Xiangyan Zhang Yadong Guo Changquan Zhang 

机构地区：[1]Department of Forensic Science,School of Basic Medical Sciences,Central South University,Changsha,China

出　　处：《Forensic Sciences Research》2022年第4期736-747,共12页法庭科学研究（英文）

基　　金：This study was supported by the National Natural Science Foundation of China[Grant No.81772026,820721140].

摘　　要：In forensic medical examinations,estimating the postmortem interval(PMI)is an important factor.Methamphetamine(MA)is a synthetic stimulant that is commonly abused,and estimation of the PMI after MA abuse has become one of the main tasks in forensic investigation.Microorganisms play a vital role in carrion decomposition.Analysing the bacterial succession patterns can be used as a forensic tool to estimate the PMI.The present study aimed to analyse bacterial succession changes during the decomposition of MA to estimate the PMI.We analysed bacterial communities in rabbits treated with three different concentrations of MA(0,22.5,and 90 mg/kg)under the natural conditions of 20°C and 70%humidity by sequencing 16S rRNA gene amplicons using the Illumina MiSeq system.We obtained 2374209 high-quality sequences and 2937 operational taxonomic units(OTUs).The relative abundances of the bacterial communities varied markedly in response to different MA concentrations.Interestingly,in response to the different concentrations of MA,Bacteroidetes became disparate in the rectum in the late PMI.Increased numbers of bacterial taxa were identified in the rectum and buccal cavity samples,except at the highest concentration of MA in the rectum samples when PMI was 0-h,than were present in live rabbits.Meanwhile,the PMI correlated significantly with bacterial succession at different taxonomic levels.Our results suggested that bacterial community succession could be used as a“microbial clock”to estimate the PMI in cases of MA-related death;however,further study is required to gain a deeper understanding of this concept.

关 键 词：Forensic sciences METHAMPHETAMINE microbes bacterial community succession postmortem interval high-throughput sequencing 

分 类 号：D919[医药卫生—法医学]