肺癌组织miR-29a-3p、TRIB2水平及其与患者病理特征、预后的关系  

Levels of miR-29a-3p and TRIB2 in lung cancer tissues and their relationship with pathological characteristics and prognosis of patients

作　　者：易宣洪 黄晓智[1] 王静[1] 李志梁[1] 熊伟[2] YI Xuanhong;HUANG Xiaozhi;WANG Jing;LI Zhiliang;XIONG Wei(Radiology and Chemistry Department Four,Tangshan People's Hospital,Tangshan 063000,China;Radiology and Chemistry Department Six,Tangshan People's Hospital,Tangshan 063000,China)

机构地区：[1]唐山市人民医院放化四科,河北唐山063000 [2]唐山市人民医院放化六科,河北唐山063000

出　　处：《东南大学学报（医学版）》2023年第3期405-411,共7页Journal of Southeast University(Medical Science Edition)

基　　金：河北省卫生健康委员会资助项目(20191600)。

摘　　要：目的:探讨微小RNA-29a-3p(miR-29a-3p)、tribbles同源蛋白2(TRIB2)在肺癌组织中的表达情况,分析其与患者临床病理特征和预后的关系。方法:选取2017年7月至2018年10月在本院进行手术治疗的80例肺癌患者的癌组织及其癌旁组织(距癌组织>5 cm)样本进行研究。采用实时荧光定量PCR技术检测肺癌组织及癌旁组织中miR-29a-3p、TRIB2 mRNA表达情况;采用Pearson相关性分析miR-29a-3p和TRIB2之间的相关性;收集肺癌患者临床病理特征,分析miR-29a-3p和TRIB2表达水平与临床病理特征的关系,采用Kaplan-Meier法分析miR-29a-3p和TRIB2表达水平与肺癌患者3年生存率的关系,采用多因素Cox回归分析肺癌患者预后影响因素。结果:肺癌组织miR-29a-3p表达低于癌旁组织,TRIB2 mRNA表达高于癌旁组织,差异具有统计学意义(P<0.05);肺癌组织miR-29a-3p和TRIB2 mRNA表达与肺癌患者TNM分期、分化程度和淋巴结转移有关(P<0.05);肺癌组织miR-29a-3p与TRIB2 mRNA表达呈负相关(r=-0.566,P<0.05);miR-29a-3p高表达组患者3年生存率(88.00%)高于低表达组患者(33.33%),TRIB2低表达组患者3年生存率(87.76%)高于高表达组患者(35.48%),差异具有统计学意义(P<0.05);肺癌患者TNM分期、分化程度、淋巴结转移、miR-29a-3p和TRIB2 mRNA水平均为患者预后影响因素(P<0.05)。结论:肺癌组织中miR-29a-3p表达下调,TRIB2 mRNA表达上调,与肺癌患者TNM分期、分化程度和淋巴结转移有关,二者表达水平可能与患者预后情况密切相关。Objective:To investigate the expression of microRNA-29a-3p(miR-29a-3p)and tribbles homologue 2(TRIB2)in lung cancer tissues,and to analyze their relationship with clinicopathological characteristics and prognosis of patients.Methods:The cancer tissues and their adjacent tissues(>5 cm away from the cancer tissues)samples from 80 patients with lung cancer who underwent surgery in our hospital from July 2017 to October 2018 were gathered for research.Real-time fluorescence quantitative PCR technology was applied to detect the expression of miR-29a-3p and TRIB2 mRNA in lung cancer tissues and adjacent tissues;Pearson correlation was applied to analyze the correlation between miR-29a-3p and TRIB2;clinicopathological characteristics of lung cancer patients were collected,the relationship between miR-29a-3p,TRIB2 expression levels and clinicopathological features was analyzed,Kaplan-Meier method was applied to analyze the relationship between the expression levels of miR-29a-3p,TRIB2 and the 3-year survival rate of lung cancer patients,multivariate Cox regression was applied to analyze prognostic factors in lung cancer patients.Results:The expression of miR-29a-3p in lung cancer tissues was lower than that in adjacent tissues,and the expression of TRIB2 mRNA was higher than that in adjacent tissues(P<0.05);the expression of miR-29a-3p and TRIB2 mRNA in lung cancer tissues was related to TNM stage,degree of differentiation and lymph node metastasis in lung cancer patients(P<0.05);there was a negative correlation between miR-29a-3p and TRIB2 mRNA expression in lung cancer tissues(r=-0.566,P<0.05);the 3-year survival rate of patients in high miR-29a-3p expression group(88.00%)was higher than that of patients in low expression group(33.33%),and the 3-year survival rate of patients in low TRIB2 expression group(87.76%)was higher than that of patients in high expression group(35.48%)(P<0.05);TNM stage,degree of differentiation,lymph node metastasis,miR-29a-3p and TRIB2 mRNA levels in lung cancer patients were all prognostic f

关 键 词：肺癌 微小RNA-29a-3p tribbles同源蛋白2 预后 

分 类 号：R734.2[医药卫生—肿瘤] R73[医药卫生—临床医学]