Assessment of the effects of intrastromal injection of adipose-derived stem cells in keratoconus patients  

作　　者：Shahrokh Ramin Ali Abbasi Masoumeh Ahadi Lina Moallemi Rad Farzad Kobarfad 

机构地区：[1]Department of Optometry,School of Rehabilitation Sciences,Incubation Center for Pharmaceutical Technology(ICPT),Shahid Beheshti University of Medical Sciences,Cell Therapy Department,Red Crescent Pharmaceutical and Clinical Complex,Tehran 1616913111,Iran [2]Student Research Committee and Department of Optometry,School of Rehabilitation Sciences,Shahid Beheshti University of Medical Sciences,Tehran 1616913111,Iran [3]Department of Molecular and Cell Biology,Faculty of Basic Sciences,University of Mazandaran,Babolsar 4561745735,Iran [4]Department of Medicinal Chemistry,Shahid Beheshti School of Pharmacy,Tehran 4516745735,Iran

出　　处：《International Journal of Ophthalmology(English edition)》2023年第6期863-870,共8页国际眼科杂志（英文版）

基　　金：the project with number IR.SBMU.RETECH.REC.1399.024 from Student Research Committee,Department of Optometry,Faculty of Rehabilitation,Shahid Beheshti University of Medical Sciences,Tehran,Iran;the“Student Research Committee”and“Research&Technology Chancellor”in Shahid Beheshti University of Medical Sciences for their financial support of this study。

摘　　要：·AIM: To evaluate the efficacy and safety of intrastromal transplantation of adipose-derived stem cells(ASCs) in keratoconus patients.·METHODS: This study was conducted on 8 eyes of 8 patients with moderate to severe keratoconus. In the patients, ophthalmic assessments including visual acuity, refraction, slit lamp examination, fundoscopy, corneal topography, and confocal microscopy were performed. Autologous stem cells were used. The isolated stem cells were injected into the corneal stroma by using femtosecond laser. Surgical procedure was similar to intracorneal ring implantation. All patients were re-assessed 1, 3, and 6mo after surgery.·RESULTS: The baseline mean visual acuity was 0.48±0.18 and improved to 0.66±0.17 after surger y and final acuity increased by 1.85±0.80 lines(P=0.001).The mean spherical refraction of patients improved 0.34 ± 0.35 D(P=0.039), and the mean cylindrical refraction of patients improved 0.84±0.23 D(P=0.016). The mean flat keratometry decreased 0.78±0.71 D(P=0.017), and the mean steep keratometry decreased 0.59±0.68 D(P=0.023). The mean central corneal thickness of patients improved of 6.29±4.47 μm(P=0.03). The mean keratocyte density at the anterior and middle stroma of cornea increased(P<0.05) but remained stable at the posterior stroma after 6mo. All patients had no complications and their corneas remained transparent. ·CONCLUSION: Intrastromal transplantation of ASCs has positive effects on vision and refractive parameters in most patients with keratoconus. After six months, visual acuity improved moderately, corneal parameters reduced slightly, and stromal keratocytes density increased. This modality is safe, and patients do not have any complications.

关 键 词：adipose-derived stem cells stromal keratocyte cellular therapy KERATOCONUS 

分 类 号：R772.2[医药卫生—眼科]