从受精卵到囊胚期胚胎在体外培养发育过程中的乳酸代谢  

Lactate metabolism from the development of zygote to blastocyst stage during culture in vitro

作　　者：千日成 邹荣良 郭陆萍 王佳佳 崔善今 张坤智 Chian Ri-Cheng;Zou Rongliang;Guo Luping;Wang Jiajia;Cui Shanjin;Zhang Kunzhi(Center for Reproductive Medicine,Shanghai 10th People's Hospital of Tongji University,Shanghai 200072,China;ARSCI Biomedical Inc,Jiaxing 314006,China;Zhejiang Center for Medical Device Evaluation,Hangzhou 310009,China)

机构地区：[1]同济大学附属上海第十人民医院生殖医学中心,上海200072 [2]艾尔斯(浙江)医学科技有限公司,嘉兴314006 [3]浙江省医疗器械审评中心,杭州310009

出　　处：《中华生殖与避孕杂志》2023年第5期530-534,共5页Chinese Journal of Reproduction and Contraception

摘　　要：体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)是将精子和卵子在体外受精结合,并在体外进行胚胎培养发育至卵裂期或囊胚期胚胎,再移植到子宫内的过程。通常把受精卵到着床前囊胚期的发育过程称之为"胚胎着床前发育"。一般认为,人的胚胎着床前发育是靠卵母细胞蓄积的蛋白和mRNA发育至8-细胞期,然后开始激活胚胎自体基因进一步发育到囊胚期。由此可见,IVF技术治疗不孕不育的重要环节之一是着床前胚胎体外培养。体外培养液的成分会直接影响着床前胚胎发育和临床治疗结局。其中,乳酸可能在受精卵早期分裂发育过程中起了相对重要作用。本文对受精卵到囊胚期胚胎着床前体外培养液的发展过程和培养液中的乳酸成分对胚胎着床前发育的影响进行概述。同时,对培养液中乳酸成分来源的乳酸盐原料进行简单的介绍。Infertility treatment with in vitro fertilization and embryo transfer(IVF-ET)involves several processes such as the production of zygote(fusion of sperm and oocyte),the culturing of embryos(from zygote to blastocyst stage),and embryo transfer to uterus.The development of culturing embryos from zygote to blastocyst stage is typically called"embryo preimplantation development".In human,it has been noted that from zygote to 8-cell stage of embryo development relies on the accumulation of proteins and mRNAs which the oocyte itself has got,and then continues to develop into the blastocyst stage after zygote genome activation(ZGA).Therefore,in vitro culture of embryos from zygote to blastocyst stage is relatively important for IVF treatment.The composition of culture media directly affects the development of embryos,and in turn,subsequently affects the outcome of the IVF treatment.Among them,lactate may play an important role in early cleavage of zygote during embryonic development cultured in vitro.In this review,we discussed the composition of developed media from zygote to blastocyst stage,dealing especially with the function of lactate in culture for embryonic development.Concurrently,we discussed commercially available materials for the lactate that are used in the making of culture media.

关 键 词：受精卵 囊胚 胚胎着床前发育 胚胎自体基因 乳酸 培养液 

分 类 号：R714.8[医药卫生—妇产科学]