商茯苓中三萜类成分的质量控制  

Quality control of triterpenoids in Shang Poria cocos

作　　者：朱庆松[1] 高歌 陈琼[1] 郭心灵 黄雅琴[1] 刁婷婷 段鸿斌[1] 朱乃亮 ZHU Qingsong;GAO Ge;CHEN Qiong;GUO Xinling;HUANG Yaqin;DIAO Tingting;DUAN Hongbin;ZHU Nailiang(Xinyang Agriculture and Forestry University,Xinyang,Henan,464000 P.R.China)

机构地区：[1]信阳农林学院,河南信阳464000

出　　处：《华西药学杂志》2023年第3期296-299,共4页West China Journal of Pharmaceutical Sciences

基　　金：信阳农林学院科技创新团队建设项目(kjcxtd-202005)。

摘　　要：目的控制商茯苓的质量。方法采用TLC法鉴别商茯苓药材中的三萜类化合物,HPLC法同时测定去氢土莫酸、猪苓酸C、去氢茯苓酸、茯苓酸等的含量。结果13.8~110.4μg·mL^(-1)去氢土莫酸、4.28~34.24μg·mL^(-1)猪苓酸C、6.24~49.92μg·mL^(-1)去氢茯苓酸、14.4~115.2μg·mL^(-1)茯苓酸与峰面积呈良好的线性关系,r分别为0.9999、0.9998、0.9999、0.9999。平均加样回收率分别为99.57%、98.83%、101.36%、100.29%,RSD分别为2.30%、2.56%、2.27%、1.82%。结论所用方法的专属性较强,能准确进行定性和定量鉴别,可用于商茯苓药材的质量控制。OBJECTIVE To control the quality of Shang Poria cocos.METHODS Identification of triterpenoids in Shang P.cocos by TLC.Simultaneous determination of dehydrotumulosic acid,polyporenic acid C,dehydropachymic acid,and pachymic acid by HPLC.RESULTS The linear response range was 13.8-110.4μg·mL^(-1)(r=0.9999)for dehydrotumulosic acid,4.28-34.24μg·mL^(-1)(r=0.9998)for polyporenic acid C,6.24-49.92μg·mL^(-1)(r=0.9999)for dehydropachymic acid and 14.4-115.2μg·mL^(-1)(r=0.9999)for pachymic acid.The average recoveries of these compounds were 99.57%(RSD=2.30%),98.83%(RSD=2.56%),101.36%(RSD=2.27%)and 100.29%(RSD=1.82%).CONCLUSION This method is fast,sensitive and reproducible.It could be used to determine dehydrotumulosic acid,polyporenic acid C,dehydropachymic acid and pachymic acid in Shang P.cocos under the same chromatogram conditions.

关 键 词：商茯苓 道地药材 去氢土莫酸 猪苓酸C 去氢茯苓酸 茯苓酸 薄层色谱 高效液相色谱 质量控制 

分 类 号：R917[医药卫生—药物分析学]