一株蔗糖偏好型海洋异养硝化-好氧反硝化菌的分离鉴定及性能研究  

Isolation,identification and characteristics of a sucrose-preferred heterotrophic nitrification-aerobic denitrification bacterium strain from seawater

作　　者：殷璐璐 吕剑[1,2] 王建华 武君[3] 张翠 YIN Lu-lu;LYU Jian;WANG Jian-hua;WU Jun;ZHANG Cui(Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China;Yantai Research Institute,Harbin Engineering University,Yantai 264006,China)

机构地区：[1]中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室,山东烟台264003 [2]中国科学院大学,北京100049 [3]哈尔滨工程大学烟台哈尔滨工程大学研究院,山东烟台264006

出　　处：《海洋环境科学》2023年第3期425-431,共7页Marine Environmental Science

基　　金：国家自然科学基金资助项目(41907152,41877131);泰山学者工程专项(tsqn201812116);中国科学院科技服务网络计划(STS计划)区域重点项目(KFJ-STS-QYZX-114)。

摘　　要：目前,养殖尾水氮含量高、无法实现达标排放是一个普遍问题,因此,亟需寻求一条低成本、高效安全的解决途径。本文从黄河三角洲海水中分离出一株蔗糖偏好型且具有异养硝化-好氧反硝化功能的菌株XH1,通过形态学观察和16S rDNA同源性分析,该菌株属于肠球菌属(Enterococcus sp.)。影响因素分析实验结果表明,该菌株在碳源为蔗糖、C/N=15、T=25℃、盐度=35的条件下,硝化反应TN去除率最高,达到78.8%;反硝化过程脱氮率最高,达到80.8%。以水产养殖广泛应用且成本低廉的蔗糖为唯一碳源,在C/N=5~30、盐度=5~55、T=15℃~35℃环境下菌株XH1均可良好生长并进行脱氮反应,这显著扩大了菌株的应用范围,为其在高氮养殖尾水处理中的应用奠定基础。At present,high nitrogen content in farming tailwater and the inability to achieve standard discharge is a common problem,so there is an urgent need to find a cost effective and safe solution.A sucrose preference strain XHl with heterotrophic nitrification-aerobic denitrification function was isolated from the coastal zone of the Yellow River Delta.Morphological observation and 16S rDNA homology analysis showed that the strain belonged to Enterococcus sp..The experiments on influencing factor analysis showed that this strain had the highest TN removal rate of 78.8%for nitrification reaction and 80.8%for denitrification reaction when sucrose used as the carbon source,C/N=15,T-25℃and salinity=35 in the medium.Strain XHl could grow well and conduct denitrification reactions when C/N=5~30,salinity=5~55,T-15℃~35℃and sucrose was used as the only carbon source in the medium.Sucrose was widely used in aquaculture because of its low cost.The results significantly expanded the scope of application of the strain XHl,and laid the foundation for its application in the treatment of high nitrogen aquaculture tailwater in practice.

关 键 词：异养硝化-好氧反硝化 蔗糖偏好型 同源性比对分析 脱氮性能 

分 类 号：X172[环境科学与工程—环境科学]