PM_(2.5)诱导肝脏脂肪变性的体外研究及其关键组分初探  

Preliminary exploration of effects and critical components for PM_(2.5)-induced hepatic steatosis in vitro

作　　者：闫志鹏 李舒月 南楠 张亚如 秦国华[1] 桑楠[1] YAN Zhipeng;LI Shuyue;NAN Nan;ZHANG Yaru;QIN Guohua;SANG Nan(College of Environment and Resource,Shanxi University,Taiyuan 030006)

机构地区：[1]山西大学环境与资源学院,太原030006

出　　处：《环境科学学报》2023年第6期494-502,共9页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.22076109,22276118)。

摘　　要：细颗粒物(PM_(2.5))暴露与非酒精性脂肪肝病(NAFLD)的发病风险增加有关.NAFLD的典型特征是肝脏脂肪变性.然而,PM_(2.5)诱导的肝脂肪变性的潜在机制和关键成分仍不清楚.本研究以HepG2细胞为体外模型,将采自我国4个城市(太原、北京、杭州和广州)的PM_(2.5)暴露细胞24 h,暴露结束后通过细胞生化指标检测、染色观察、荧光定量PCR技术及皮尔森相关性检验评估PM_(2.5)及其化学组分对肝细胞脂质积累的影响.结果发现,不同城市的PM_(2.5)均以剂量依赖的方式降低了细胞活力,广州市PM_(2.5)的细胞毒性相对较强.此外,同等浓度下(30μg·mL^(-1)),广州市PM_(2.5)暴露细胞引起的脂质积累现象明显高于对照组及其他城市暴露组,而北京市和杭州市PM_(2.5)暴露细胞24 h并未引起明显的细胞脂质积累.相关性分析结果显示,钒(V)、铜(Cu)、砷(As)、锡(Sn)、锑(Sb)及钨(W)与至少两种肝细胞脂质积累指标(甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL))呈显著正相关.上述结果表明,广州市PM_(2.5)诱导HepG2细胞发生脂质积累最为明显,Cu、As、Sn、Sb和W等无机组分很可能是PM_(2.5)中诱导HepG2细胞脂肪变性的关键毒性组分.It has been reported that fine particulate matter(PM_(2.5))exposure is associated with an increased risk of nonalcoholic fatty liver disease(NAFLD).NAFLD is typically characterized by hepatic steatosis.However,the underlying mechanism and critical components of PM_(2.5)-induced hepatic steatosis remain unclear.In this study,we investigated the cytotoxicity of PM_(2.5)from four cities in China(Taiyuan,Beijing,Hangzhou,and Guangzhou)in HepG2 cells.The effects of PM_(2.5)and its chemical components on lipid accumulation in HepG2 cells were evaluated by cell biochemical indicators detection,staining observation,quantitative real-time PCR assay,and Pearson correlation test.The results showed that PM_(2.5)from different cities decreased cell viability in a dose-dependent manner.In addition,Guangzhou PM_(2.5)caused the most severe lipid accumulation,while PM_(2.5)from Beijing and Hangzhou did not cause noticeable effects at the same concentration.The results of correlation analysis showed a significant positive correlation between vanadium(V),copper(Cu),arsenic(As),tin(Sn),antimony(Sb),tungsten(W)and at least two of the four lipid accumulation indicators(triglyceride(TG),total cholesterol(TC),low-density lipoprotein(LDL),and very low-density lipoprotein(VLDL)).The above results showed that Guangzhou PM_(2.5)induced the most obvious lipid accumulation,and the inorganic components such as Cu,As,Sn,Sb,and W attached to PM_(2.5)were probably the critical components to induce hepatic steatosis in HepG2 cells.

关 键 词：PM_(2.5) HEPG2细胞 脂质积累 关键组分 

分 类 号：X171.5[环境科学与工程—环境科学]