Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ reduced silver nanoparticles for wound healing  

作　　者：Na Xu Yucheng Yuan Liangping Ding Jiangfeng Li Jiezhi Jia Zheng Li Dengfeng He Yunlong Yu 

机构地区：[1]Institute of Burn Research,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,400038,China [2]School of Materials science and Engineering,Xihua University,No.999,Jinzhou Road,Jinniu District,Chengdu City,Sichuan Province,Chengdu,610039,China

出　　处：《Burns & Trauma》2022年第1期401-415,共15页烧伤与创伤（英文）

基　　金：support from the National Natural Science Foundation of China(51703145);China Postdoctoral Science Foundation(2019 T120844 and 2017 M620426);Sichuan Science and Technology Program(2021JDRC0102);Natural Science Foundation of Chongqing(cstc2021jcyj-msxmX0513);supported by the National Natural Science Foundation of China(51703145);Natural Science Foundation of Chongqing(cstc2021jcyj-msxmX0513).

摘　　要：Background:Most traditional wound dressings only partially meet the needs of wound healing because of their single function.Patients usually suffer from the increasing cost of treatment and pain resulting from the frequent changing of wound dressings.Herein,we have developed a mutifunctional cryogel to promote bacterial infected wound healing based on a biocompatible polysaccharide.Methods:The multifunctional cryogel is made up of a compositive scaffold of chitosan(CS),gelatin(Gel)and tannic acid(TA)and in situ formed silver nanoparticles(Ag NPs).A liver bleeding rat model was used to evaluate the dynamic hemostasis performance of the various cryogels.In order to evaluate the antibacterial properties of the prepared cryogels,gram-positive bacterium Staphylococcus aureus(S.aureus)and gram-negative bacterium Escherichia coli(E.coli)were cultured with the cryogels for 12 h.Meanwhile,S.aureus was introduced to cause bacterial infection in vivo.After treatment for 2 days,the exudates from wound sites were dipped for bacterial colony culture.Subsequently,the anti-inflammatory effect of the various cryogels was evaluated by western blotting and enzyme-linked immunosorbent assay.Finally,full-thickness skin defect models on the back of SD rats were established to assess the wound healing performances of the cryogels.Results:Due to its porous structure,the multifunctional cryogel showed fast liver hemostasis.The introduced Ag NPs endowed the cryogel with an antibacterial efficiency of>99.9%against both S.aureus and E.coli.Benefited from the polyphenol groups of TA,the cryogel could inhibit nuclear factor-κB nuclear translocation and down-regulate inflammatory cytokines for an anti-inflammatory effect.Meanwhile,excessive reactive oxygen species could also be scavenged effectively.Despite the presence of Ag NPs,the cryogel did not show cytotoxicity and hemolysis.Moreover,in vivo experiments demonstrated that the biocompatible cryogel displayed effective bacterial disinfection and accelerated wound healing.Conclusions:The mu

关 键 词：Wound dressing CRYOGEL Bacterial infection ANTI-INFLAMMATION HEMOSTASIS Antibacterial Wound healing 

分 类 号：R318.08[医药卫生—生物医学工程]