复方守宫散通过TNF-α信号通路对骨癌痛大鼠痛觉敏化机制的研究  

Effect of Compound Shougong Powder on Pain Sensitization in Rats with Bone Cancer Pain through TNF-α Signaling Pathway

作　　者：蔡琪 张良军 黄璟 毛汉文 田敬海 夏黎明 CAI Qi;ZHANG Liangjun;HUANG Jing;MAO Hanwen;TIAN Jinghai;XIA Liming(The First Clinical Medical College,Anhui Medical University,Hefei,230037,China;Department of Oncology,Wuhan Dongxihu District People’s Hospital,Wuhan,430040,China;Department of Orthopedics,Wuhan Dongxihu District People’s Hospital,Wuhan,430040,China;Department of Oncology,First Affiliated Hospital of Anhui Medical University,Hefei,230031,China)

机构地区：[1]安徽医科大学第一临床学院,合肥市230037 [2]武汉市东西湖区人民医院肿瘤科,武汉市430040 [3]武汉市东西湖区人民医院骨外科,武汉市430040 [4]安徽医科大学第一附属医院肿瘤科合肥市,230031

出　　处：《医学分子生物学杂志》2023年第4期346-350,358,共6页Journal of Medical Molecular Biology

基　　金：武汉市医学科研项目(No.WZ20A02)。

摘　　要：目的探讨复方守宫散(Compound Shougong powder,CSP)通过肿瘤坏死因子α(TNF-α)信号通路对骨癌痛大鼠痛觉敏化机制的影响。方法通过注射Lewis肺癌细胞建立肺癌骨癌痛大鼠模型;将大鼠随机分为模型组、阳性对照(扶他林,0.1 g/kg)组、CSP低(CSP-L,20 mg/kg)、中(CSP-M,40 mg/kg)、高(CSP-H,80 mg/kg)剂量组,并设置假手术组大鼠,每组各10只。干预结束后,检测大鼠机械性缩足反射阈值(paw withdrawal mechanical threshold,PWMT);全自动热痛测试仪检测大鼠热痛觉缩足潜伏期(paw withdrawal thermal lathency,PWTL);ELISA法检测血清中TNF-α、白介素(IL)-1β、IL-6水平变化;Western印迹检测各组大鼠脊髓组织单核细胞趋化蛋白-1(MCP-1)以及TNF-α表达水平。结果与假手术组相比,模型组大鼠IL-1β、IL-6水平、TNF-α、MCP-1表达均显著升高,PWMT显著降低,PWTL显著缩短(P<0.05);与模型组相比,CSP-L组、CSP-M组、CSP-H组大鼠IL-1β、IL-6水平、TNF-α、MCP-1表达均显著降低,PWMT显著升高,PWTL显著延长(P<0.05);CSP-H组各项指标与阳性对照组差异无统计学意义(P>0.05)。结论CSP可以改善肺癌骨癌痛大鼠疼痛反应,发挥止痛作用,可能与抑制TNF-α信号通路有关。Objective To investigate the effect of Compound Shougong powder(CSP)on the pain sensitization in rats with bone cancer pain through tumor necrosis factor-α(TNF-α)signaling pathway.Methods A rat model of lung cancer bone metastases pain was established by injecting Lewis lung cancer cells.Rats were randomly divided into 6 groups:model group,positive control group(Voltaren,0.1 g/kg),CSP low-dose group(CSP-L,20 mg/kg),CSP medium-dose group(CSP-M,40 mg/kg),CSP high-dose group(CSP-H,80 mg/kg),and the sham operation group,10 rats in each group.After the intervention,the paw withdrawal mechanical threshold(PWMT)of the rats was detected,and the paw withdrawal thermal latency(PWTL)in rats was detected by the automatic thermal pain tester.The levels of TNF-α,IL-1βand IL-6 in serum were detected by ELISA.Western blotting was performed to detect the expression levels of monocyte chemoattractant protein-1(MCP-1)and TNF-αin spinal cord tissues of rats in each group.Results The levels of IL-1β,IL-6,TNF-αand MCP-1 in the model group were significantly increased when compared with those in the sham operation group,while the values of PWMT and PWTL were significantly decreased(P<0.05).The levels of IL-1β,IL-6,TNF-αand MCP-1 in the CSP-L group,CSPM group and CSP-H group were significantly decreased when compared with those in the model group,while the values of PWMT and PWTL were significantly increased(P<0.05).No obvious differences in the above indexes were found between the CSP-H group and the positive control group(P>0.05).Conclusion CSP may alleviate the pain response in rats with lung cancer bone metastases pain and exert analgesic effect by inhibiting the expression of TNF-αand other inflammatory factors.

关 键 词：复方守宫散 TNF-Α 肺癌 骨癌痛 痛觉敏化 

分 类 号：R743.3[医药卫生—神经病学与精神病学]