苦荞FtCIPK的鉴定及响应干旱高钙胁迫的表达分析  

Identification of FtCIPK in Fagopyrum tataricum and Gene Expression Responding to Drought and High Calcium Stress

作　　者：关志秀 韦春玉 李梅 任兵 任欢欢 汪燕 梁成刚 GUAN Zhixiu;WEI Chunyu;LI Mei;REN Bing;REN Huanhuan;WANG Yan;LIANG Chenggang(Research Center of Buckwheat Industry Technology,College of Life Sciences,Guizhou Normal University,Guiyang 550001,China)

机构地区：[1]贵州师范大学生命科学学院荞麦产业技术研究中心,贵阳550001

出　　处：《四川农业大学学报》2023年第3期399-408,共10页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(32060511、32160428);贵州省科技支撑项目(黔科合ZC[2019]2298);贵州省高层次创新型千层次人才项目(111/0920008)。

摘　　要：【目的】鉴定苦荞(Fagopyrum tartaricum L.)类钙调磷酸酶B互作蛋白激酶(FtCIPK),并研究干旱与高钙下FtCIPK的表达量变化,为探究苦荞耐干旱高钙的机理与FtCIPK基因功能提供科学参考。【方法】利用生物信息学方法对苦荞FtCIPK基因进行分析,并模拟干旱、高钙和干旱高钙胁迫研究FtCIPK基因的表达模式。【结果】在苦荞8条染色体上共鉴定到5个FtCIPK基因,分别定位于染色体Ft3(2个)、Ft5(1个)和Ft6(2个),cDNA长度为1323~1467 bp,编码氨基酸440~488个,序列高度保守,均含蛋白激酶和NAF结构域。干旱下FtCIPK11在8 h、24 h显著下调,FtCIPK14在24 h极显著下调,FtCIPK10在24 h极显著上调;高钙下FtCIPK11在8 h、24 h极显著下调;FtCIPK10在8 h极显著上调,24 h显著下调;FtCIPK14在8 h极显著下调,24 h极显著上调;FtCIPK5在24 h极显著下调;干旱高钙下FtCIPK10和FtCIPK11在8 h、24 h显著下调,FtCIPK12在8 h、24 h极显著上调,FtCIPK14在24 h显著上调。【结论】说明FtCIPK5能响应高钙,FtCIPK10、FtCIPK11、FtCIPK14能响应干旱、高钙和干旱高钙,FtCIPK12能响应干旱高钙胁迫。相关性分析推测FtCIPK5、FtCIPK10、FtCIPK12、FtCIPK14可与FtCBL3-1,FtCIPK11可与FtCBL1和Ft CBL9,FtCIPK10和FtCIPK14可与FtCBL2互作响应干旱与高钙胁迫。【Objective】This study aims to provide scientific reference for exploring the mechanism of drought and high calcium tolerance.of calcineurin B-like protein kinase gene(FtCIPK)in Tartary buckwheat(Fagopyrum tartaricum L.).【Method】Tartary buckwheat FtCIPK genes were analyzed by employing bioinformatics method.The drought stress(15%PEG),high calcium stress(0.15 mol/L CaCl2)and combined drought and high calcium stress(15%PEG+0.15 mol/L CaCl2)were performed to analyze the gene expression pattern of FtCIPK gene under abiotic stresses.【Result】A total of five FtCIPK genes in Tartary buckwheat were identified,and they were located on chromosomes Ft3(2),Ft5(1)and Ft6(2),respectively,by screening of 8 chromosomes.The cDNA length of FtCIPK were 1323-1467 bp,encoding 440-488 amino acids,of which protein sequences were highly conserved and contained protein kinase and NAF domain.Under drought stress,FtCIPK11 was significantly down-regulated at 8 h and 24 h,FtCIPK14 was significantly down-regulated at 24 h,and FtCIPK10 was significantly up-regulated at 24 h.Under high calcium stress FtCIPK11 was significantly down-regulated at 8 h and 24 h,FtCIPK10 was significantly up-regulated at 8 h but down-regulated at 24 h,FtCIPK14 was significantly downdoiregulated at 8 h but up-regulated at 24 h,and FtCIPK5 was significantly down-regulated at 24 h.Under combined drought and high calcium stress,FtCIPK10 and FtCIPK11 were significantly down-regulated at 8 h and 24 h,FtCIPK12 was significantly up-regulated at 8 h and 24 h,and FtCIPK14 was significantly up-regulated at 24 h.【Conclusion】These results indicated that FtCIPK5 was induced by high calcium stress.FtCIPK10,FtCIPK11 and FtCIPK14 were induced by drought,high calcium and drought high calcium stress.FtCIPK12 was induced by combined drought and high calcium stress.Correlation analysis of gene expression implied that FtCIPK5,FtCIPK10,FtCIPK12,FtCIPK14 might interact with FTCBL3-1,and FtCIPK11 might interact with FtCBL1 and FtCBL9,FtCIPK10 and FtCIPK14 might interact with F

关 键 词：苦荞 类钙调磷酸酶B互作蛋白激酶 干旱 高钙 基因表达 

分 类 号：S517[农业科学—作物学]