贮藏温度对茶树精油中烯醇类化合物代谢规律的影响  

Effects of Different Storage Temperature on Metabolism Pathway of Terpenes and Terpineols in Tea Tree Oil

作　　者：刘义军[1,2] 赵雨诗 袁源 廖雪汝[1,2] 林丽静 陆建能 LIU Yijun;ZHAO Yushi;YUAN Yuan;LIAO Xueru;LIN Lijing;LU Jianneng(Hainan Key Laboratory of Storage&Processing of Fruits and Vegetables,Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524001,Guangdong,China;Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs,Zhanjiang 524001,Guangdong,China;College of Tropical Crops Institute,Yunnan Agricultural University,Pu′er 665099,Yunnan,China)

机构地区：[1]中国热带农业科学院农产品加工研究所,海南省果蔬贮藏与加工重点实验室,广东湛江524001 [2]农业农村部热带作物产品加工重点实验室,广东湛江524001 [3]云南农业大学热带作物学院,云南普洱665099

出　　处：《四川农业大学学报》2023年第3期425-429,470,共6页Journal of Sichuan Agricultural University

基　　金：海南省自然科学基金高层次人才(321RC634);新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划)(2022E01023)。

摘　　要：【目的】揭示贮藏温度对茶树精油中烯醇类化合物代谢规律的影响,为提升茶树精油品质提供数据支撑。【方法】采用水蒸气蒸馏法从互叶白千层的嫩枝叶提取精油,采用气相色谱-质谱联用仪对茶树精油中烯醇类化合物进行检测。【结果】随着贮藏时间的延长,不同贮藏温度下,松油烯-4-醇、α-松油烯与γ-松油烯相对含量的变化趋势为先上升后下降,异松油烯与α-松油醇相对含量变化为先下降后上升,其中35℃对烯醇类化合物相对含量变化影响较明显。采用Pearson相关系数法对松油烯-4-醇、α-松油烯与γ-松油烯等化合物进行相关性分析,并结合化合物共轭效应等有机化学相关原理,揭示了松油烯-4-醇在贮藏过程烯醇类化合物的代谢路径,为指导茶树精油生产和贮藏提供了理论支撑。【结论】不同贮藏温度均能影响茶树精油中烯醇类化合物的含量,控制好贮藏温度对提升茶树精油品质具有重要作用。【Objective】The current study was conducted to reveal the influence of storage temperature of tea tree oil on the metabolism of terpenes and terpineols during storage finally aiming at providing data support for improving the quality of tea tree oil.【Method】Tea tree oil was extracted from the branches and leaves of Melaleuca alternifolia by steam distillation,and terpenes and terpineols in tea tree oil were detected by using gas chromatography mass spectrometry(GC-MS).【Result】The relative contents of terpinene-4-ol,α-terpinene andγ-terpinene increased first and then decreased,while those of terpinene andα-terpineol decreased first and then increased,among which 35℃had significant influence on the relative contents of terpene and terpineol compounds.Pearson correlation coefficient method was used to analyze the correlation of terpinene-4-ol,α-terpinene andγ-terpinene.The metabolic pathway of terpene and terpineol compounds of terpinene-4-ol during storage was revealed by combining the organic chemistry related principles such as compound conjugation effect,which provided theoretical support for guiding the production and storage of tea tree oil.【Conclusion】Different storage temperatures could affect the content of terpenes and terpineols in tea tree oil.Controlling the storage temperature playes an important role in improving the quality of tea tree oil.

关 键 词：茶树精油 烯类化合物 醇类化合物 气相色谱-质谱法 贮藏温度 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]