Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles  

作　　者：Duanping Sun Ying Ma Maoqiang Wu Zuanguang Chen Luyong Zhang Jing Lu 

机构地区：[1]Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances,Center for Drug Research and Development,Guangdong Pharmaceutical University,Guangzhou,510006,China [2]Key Specialty of Clinical Pharmacy,The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,510699,China [3]National and Local United Engineering Lab of Druggability and New Drugs Evaluation,School of Pharmaceutical Sciences,Sun Yat-Sen University,Guangzhou,510006,China

出　　处：《Journal of Pharmaceutical Analysis》2023年第4期340-354,共15页药物分析学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Nos.:82003710 and 82173808);the Natural Science Foundation of Guangdong Province(Grant Nos.:2020A1515010075 and 2021B1515020100);the Project of Educational Commission of Guangdong Province(Grant No.:2021ZDZX2012);the Guangzhou Basic and Applied Basic Research Project(Grant No.:2023A04J1163);the National Key Clinical Specialty Construction Project(Clinical Pharmacy);High-Level Clinical Key Specialty(Clinical Pharmacy)in Guangdong Province,China.

摘　　要：Liquid biopsy is a technology that exhibits potential to detect cancer early,monitor therapies,and predict cancer prognosis due to its unique characteristics,including noninvasive sampling and real-time analysis.Circulating tumor cells(CTCs)and extracellular vesicles(EVs)are two important components of circulating targets,carrying substantial disease-related molecular information and playing a key role in liquid biopsy.Aptamers are single-stranded oligonucleotides with superior affinity and specificity,and they can bind to targets by folding into unique tertiary structures.Aptamer-based microfluidic platforms offer new ways to enhance the purity and capture efficiency of CTCs and EVs by combining the advantages of microfluidic chips as isolation platforms and aptamers as recognition tools.In this review,we first briefly introduce some new strategies for aptamer discovery based on traditional and aptamer-based microfluidic approaches.Then,we subsequently summarize the progress of aptamer-based microfluidics for CTC and EV detection.Finally,we offer an outlook on the future directional challenges of aptamer-based microfluidics for circulating targets in clinical applications.

关 键 词：APTAMER Microfluidic Circulating tumor cells Extracellular vesicles BIOANALYSIS 

分 类 号：R392-33[医药卫生—免疫学]