梁王茶多糖的纯化及体外抗氧化作用研究  

Study on purification and antioxidant activity of polysaccharide from nothopanax delavayi

作　　者：沈晓静 周绍琴 王秋萍[3] 李泽林 解富娟 冯宇 邵俊文 龚姝淳 字成庭 SHEN Xiao-jing;ZHOU Shao-qin;WANG Qiu-ping;LI Ze-lin;XIE Fu-juan;FENG Yu;SHAO Jun-wen;GONG Shu-chun;ZI Cheng-ting(College of Science,Yunnan Agricultural University,Kunming 650201,China;Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming,650500,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学理学院,云南昆明650201 [2]云南省天然药物药理重点实验室,昆明医科大学,云南昆明650500 [3]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《化学研究与应用》2023年第6期1350-1355,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(32260090)资助;云南省天然药物药理重点实验室开放研究基金项目(70120030506)资助;云南省重大科技专项子课题项目(202002AE09001004)资助。

摘　　要：为进一步研究化梁王茶多糖,对其进行纯化后评价其体外抗氧化活性。采用Sevag法、AB-8大孔树脂、DEAE-52纤维素、凝胶分离技术对梁王茶粗多糖进行纯化;测定纯化前后多糖中多糖、糖醛酸、蛋白质、总酚和总黄酮含量变化;运用傅里叶变换红外光谱(fourier transform infrared spectroscopy,FT-IR)和扫描电镜(scan-ning electron microscope,SEM)表征其结构特点;再通过DPPH自由基清除实验和FRAP法评估其抗氧化能力。结果表明,通过纯化梁王茶多糖中的蛋白质、总酚、总黄酮被大量除去,呈现清晰的中空球体状结构,并表现了一定的抗氧化活性。纯化后多糖的DPPH自由基清除能力增强,IC50值由7.274 mg·mL^(-1)降低为7.101 mg·mL^(-1);而FRAP抗氧化能力则略有降低,抗氧化能力由0.342 mmol Fe^(2+)/g降低为0.237 mmol Fe^(2+)/g。因此,梁王茶多糖可作为一种天然活性多糖具有潜在的开发价值和深入研究的意义。To purifiy and evaluate antioxidant activity of polysaccharide from nothopanax delavayi,Sevag method,AB-8 macroporous resin,DEAE-52 cellulose column,and gel were used to purify rude polysaccharide of nothopanax delavayi.Their polysaccharides,uronic acids,protein,total polyphenols,and total flavonoids contents were measured.Then their structural characteristics were characterized by fourier transform infrared spectroscopy(FT-IR)and scanning electron microscope(SEM).Moreover,their antioxidant capacities were evaluated by DPPH radical scavenging assay and FRAP method.The results showed that purified polysaccharides of nothopanax delavayi had a clear hollow spheroid structure,and the purification of rude polysaccharides was effective.The results of antioxidant assays showed that polysaccharides of nothopanax delavayi had certain antioxidant activities.And the DPPH radical scavenging capacity of purified polysaccharide was higher than rude polysaccharide,the values of IC50 changed from 7.274 mg·mL^(-1)to 7.101 mg·mL^(-1).While the ferric reducing antioxidant power were lower than rude polysaccharide,the antioxidant activities changed from 0.342 mmol Fe^(2+)/g to 0.237 mmol Fe^(2+)/g.Therefore,nothopanax delavayi polysaccharides could be used as the source of natural active polysaccharide,which had the potential development value and the significance of further study.

关 键 词：梁王茶 多糖 纯化 抗氧化活性 理化性质 

分 类 号：O629.3[理学—有机化学]