基于QuEChERS-超临界流体色谱-串联质谱法的茶叶中甲胺磷及乙酰甲胺磷对映体拆分及定量  

Separation and Quantification of Methamidophos and Acephate Enantiomer in Tea Based on QuEChERS-Supercritical Fluid Chromatography-Tandem Mass Spectrometry

作　　者：王丽 WANG Li(Beijing Institute of Food Inspection and Research(Beijing Food Safety Monitoring and Risk Assessment Center),Beijing 100094)

机构地区：[1]北京市食品检验研究院(北京市食品安全监控和风险评估中心),北京100094

出　　处：《安徽农业科学》2023年第11期156-162,170,共8页Journal of Anhui Agricultural Sciences

摘　　要：采用QuEChERS法提取,在以Chiral-Pak IC-3为色谱柱的超临界流体色谱上拆分,大气压化学电离后串联四极杆质谱法测定。结果表明,茶叶中(+)-甲胺磷、(+)-乙酰甲胺磷的检出限为5μg/kg,定量限为10μg/kg,平均回收率为70.53%~110.61%,相对标准偏差(RSD)为2.92%~13.30%。该方法能够有效拆分和定量甲胺磷和乙酰甲胺磷对映异构体。Extraction was performed by QuEChERS method,separated on supercritical fluid chromatography using Chiral Pak IC-3 as the chromatographic column,and determined by atmospheric pressure chemical ionization followed by tandem quadrupole mass spectrometry.The result showed that the detection limit of(+)-methamidophos and(+)-acephate in tea was 5μg/kg,the limit of quantification was 10μg/kg.The average recoveries were 70.53%-110.61%and RSD were 2.92%-13.30%.This method can effectively separate and quantify methamidophos and acephate enantiomer.

关 键 词：QUECHERS 超临界流体色谱-串联质谱 茶叶 甲胺磷 乙酰甲胺磷 对映异构体 

分 类 号：TS272[农业科学—茶叶生产加工]