复配高效菌群厌氧降解煤产甲烷实验研究  

Experimental study on methane generation from anaerobic degradation of coal by compound high-efficiency flora

作　　者：弓凯仪 郭红光 张益瑄[1,2] 张佳艳 GONG Kaiyi;GUO Hongguang;ZHANG Yixuan;ZHANG Jiayan(College of Safety and Emergency Management Engineering,Taiyuan University of Technology,Taiyuan 030024,China;Key Laboratory of In-Situ Property-Improving Mining of Ministry of Education,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学安全与应急管理工程学院,山西太原030024 [2]太原理工大学原位改性采矿教育部重点实验室,山西太原030024

出　　处：《矿业安全与环保》2023年第3期12-17,22,共7页Mining Safety & Environmental Protection

基　　金：国家自然科学基金项目(U1810103,51404163);山西省重点研发计划项目(国际合作项目)(201903D421088);山西省煤层气联合基金项目(2014012006)。

摘　　要：高效功能菌群是微生物增产煤层气技术的重要影响因素。通过复配降解芳香化合物的功能真菌菌群与降解煤产甲烷的真菌—产甲烷菌群,提高甲烷产量;分析菌群结构、中间产物和煤结构的演替规律,研究复配菌群降解褐煤产甲烷机理。研究结果表明:复配菌群能够厌氧降解褐煤,煤的甲烷产量为172μmol/g,是未复配的1.74倍,芳烃降解菌Cladosporium在第7天成为优势真菌属;具有较强环境适应性的Aspergillus、Penicillium等成为产气结束后的优势真菌,在第7天发酵液中检测到丰富的代谢中间产物脂肪酸和芳香酸,其占比在产气末期均下降50%以上;煤中芳香碳在降解后下降了12.27%,降解效果显著。研究结果为改善产甲烷菌群的煤降解能力,增强产甲烷效能提供了一种有效方法。High-efficiency functional flora is an important factor for microbially enhanced coalbed methane.In this paper,the functional fungal flora that degrades aromatic compounds and the fungus-methanogenic flora that degrades coal to produce methane are combined to improve methane production.The succession law of flora structure,intermediate product and coal structure was analyzed,and the mechanism of methanogenesis from lignite degraded by compound flora was studied.The results show that the compound flora can anaerobically degrade the lignite,and the methane production of the coal is 172μmol/g,which is 1.74 times higher than that none-compound flora.The aromatic degrading fungi Cladosporium become the dominant fungal genus on the 7th day.Aspergillus and Penicillium,which have strong environmental adaptability,become the dominant fungi after gas production.Abundant metabolic intermediate fatty acids and aromatic acids are detected in the fermentation broth on the 7th day,and their proportions decreases by more than 50%at the end of gas production.Aromatic carbon in the coal decreases by 12.27%after degradation,which is significant.The research results provide an effective method for improving the coal degradation ability of methanogenic bacteria and enhancing methanogenesis efficiency.

关 键 词：微生物增产煤层气 厌氧降解 生物强化 复配 核磁 GC-MS&LC-MS 

分 类 号：TD712[矿业工程—矿井通风与安全] X172[环境科学与工程—环境科学]