白细胞介素-1β通过IL-6/JAK2/STAT3轴促进脑胶质瘤细胞的侵袭  

IL-1βpromotes the invasion of glioma cells through the IL-6/JAK2/STAT3 axis

作　　者：王棒 吴娟[1] 陈硕硕 程哲 束汉生[1] WWANG Bang;WU Juan;CHEN Shuo Shuo(Department of Neurosurgery,The Second Af filiated Hospital of Bengbu Medical College,Beng 233000,China)

机构地区：[1]蚌埠医学院第二附属医院神经外科,安徽蚌埠233000

出　　处：《中国实验诊断学》2023年第5期596-602,共7页Chinese Journal of Laboratory Diagnosis

基　　金：安徽省高校自然科学研究项目(KJ2021A0786);蚌埠医学院研究生科研创新计划项目(Byycxz21079)。

摘　　要：目的探讨白细胞介素-1β(IL-1β)在脑胶质瘤细胞侵袭中的作用及其机制。方法使用ELISA检测IL-1β和IL-6在人星形胶质细胞(NHA)、U87、U251、A172胶质瘤细胞培养液上清中的浓度,根据结果挑选实验细胞,随后检测IL-1β刺激下的IL-6生成。在不同浓度的IL-1β刺激、改变IL-6含量、下调JAK2和STAT3基因表达水平等不同条件下,使用Transwell小室实验检测胶质瘤细胞侵袭能力的变化以及Western blot检测JAK2、STAT3磷酸化蛋白水平的改变。结果与NHA相比,U87、U251的培养液中IL-1β水平明显升高,U87、U251、A172的培养液中IL-6水平均明显升高。在U87、U251细胞中,IL-1β不仅能促进细胞的侵袭,而且能促进其IL-6的产生。在IL-1β的刺激下,IL-6蛋白能进一步促进细胞的侵袭,而IL-6中和抗体则明显抑制IL-1β的促侵袭作用。研究表明IL-1β刺激可促进U87、U251细胞JAK2和STAT3的磷酸化蛋白表达。在IL-1β的刺激下,IL-6蛋白能进一步促进它们的表达,而IL-6抗体则明显抑制其表达。同时,下调JAK2和STAT3也能显著抑制IL-1β刺激下的U87、U251细胞的侵袭能力。结论IL-1β通过IL-6/JAK2/STAT3轴促进脑胶质瘤细胞的侵袭。ObjectiveeTo investigate the role of interleukin-1p(IL-1p)in glioma cells.Methods The ELISA was used to measure the concentrations of IL-1βand IL-6 in the supernatant of NHA,U87,U251 and A172 cell cultures,experimental cells were selected according to the results.Then IL-6 production in response to IL-1βstimulation was subsequently measured.Transwell and western blot assays were used to detect the invasion of glioma cells,as well as changes of JAK2 and STAT3 protein phosphorylation levels,under the treatment conditions of stimulation with different concentrations of IL-1p,changing IL-6 content,and down-regulation of the expression levels of the JAK2 and STAT3 genes.Results Compared with NHA,the level of IL-1p in U87 and U251 culture medium was significantly increased.The level of IL-6 in culture medium of U87,U251 and A172 was significantly increased.In U87 and U251 cells,IL-1βpromoted the invasion of glioma cells,and the production of IL-6.Under the stimulation of IL-1p,IL-6 protein can further promote the invasion of glioma cells,while IL-6 antibody can significantly inhibit it.Studies showed that IL-1β,through IL-6,affected the expressions of JAK2 and STAT3 phosphorylated proteins in U87 and U251 cells.The downregulation of JAK2 and STAT3 significantly inhibited the invasion of U87 and U251 cells stimulated by IL-1p.Conclusion IL-1βaffects the invasion of glioma cells through the IL-6/JAK2/STAT3 axis.

关 键 词：脑胶质瘤 侵袭 IL-1Β IL-6/JAK2/STAT3 

分 类 号：R739.41[医药卫生—肿瘤]