LncRNA、AFAP1-AS1表达与重症肌无力发病相关性研究  

Study on the correlation between the expression of IncRNA、AFAP1-AS1 and myasthenia gravis

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作  者:孔晓彤 蔡涵璐 王宇[1] 王健健 张荟雪[1] 王丽华[1] Kong Xiaotong;Cai Hanlu;Wang Yu;Wang Jianjian;Zhang Huixue;Wang Lihua(Department of Neurology,the 2nd Afiliated Hospital,Harbin Medical University,Heilongjiang 150086,China)

机构地区:[1]哈尔滨医科大学附属第二临床医院神经内科,黑龙江150086

出  处:《脑与神经疾病杂志》2023年第6期354-358,共5页Journal of Brain and Nervous Diseases

基  金:国家自然科学基金重点国际合作研究项目(81820108014);中国博士后课题(2020M681114);黑龙江省博士后课题(LBH-Z19181)。

摘  要:目的探讨lncRNA肌动蛋白丝相关蛋1-反义RNA1(AFAP1-AS1)在重症肌无力(MG)患者中的表达水平及其临床意义。方法收集2020年1月至2021年12月期间收治的35例MG患者和35例健康体检者的外周静脉血,提取外周血单个核细胞(PBMCs)中的总RNA,应用RT-PCR方法检测AFAP1 AS1的表达水平,并进一步分析在MG各亚组中AFAP1 AS1的表达水平,并分析AFAP1-AS1与定量MG评分(QMGS)之间的相关性。结果MG患者PBMCs中AFAP1-AS1的表达水平(1.498±1.136)显著高于健康对照组(0.619±0.707),差异有统计学意义(Z=-3.46,P=0.001)。AFAP1-AS1的表达水平在胸腺增生亚组(2.212±0.314)中明显高于胸腺正常组(1.076±0.197),差异有统计学意义(t=3.228,P=0.0028),在性别亚组及早发晚发亚组中差异无统计学意义。且AFAP1-AS1与QMGS评分之间不具有相关性。结论AFAP1-AS1在MG患者的PBMCs中表达水平明显上调,可视为MG新的分子诊断标志物。Objective To investigate the expression level and clinical significance of actin filament-associated protein 1-antisense RNA1(AFAP1-AS1)in myasthenia gravis(MG).Methods We collected blood samples of all participant from January 2020 to December 2021.The total RNA was extracted from peripheral blood mononuclear cells(PBMCs).The expression level of AFAP1-AS1 in PBMCs was detected by RT-PCR.Furthermore,the association between AFAP1-AS1 expression level and quantitative myasthenia gravis score(QMGS)was analyzed.Results The expression level of AFAP1-AS1 in PBMCPs was significantly upregulated in MG patients(1.498±1.136)compared with healthy control group(0.619±0.707).In thymic hyperplasia subgroup,the expression level of AFAP1-AS1 was significantly upregulated(2.212±0.314)compared with normal thymus MG patients(1.076±0.197).However,there was no significant difference in AFAP1-AS1 expression in MG other subgroups,and no correlation with QMGS.Conclusion The expression level of AFAP1-AS1 was increased in MG patients,which might be a new molecular biomarker and therapeutic target in MG.

关 键 词:重症肌无力 外周血单个核细胞 长链非编码RNA 肌动蛋白丝相关蛋白1-反义RNA1 

分 类 号:R746.1[医药卫生—神经病学与精神病学]

 

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